摘要目的 研究EB病毒(Epstein-Barr virus,EBV)感染肝硬化患者对T细胞免疫球蛋白及黏蛋白域蛋白3(T cell immunoglobulin-and mucin-domain-containing molecule-3,Tim-3)和程序性细胞死亡蛋白1(programmed death-1,PD-1)以及细胞外调节蛋白激酶(extracellular regulated protein kinases,ERK)影响.方法 本次研究筛选2017年8月至2018年8月收治的47例乙型肝炎肝硬化的患者作为研究对象,根据EBV感染情况分为EBV感染组和无EBV感染组,比较两组血清Tim-3、PD-1、ERK蛋白表达情况.结果 两组间外周单核细胞CD14++CD16-亚群、CD14++CD16+亚群及NK细胞中PD-1、Tim-3表达的差异均有统计学意义.EBV感染组ERK蛋白表达阳性率为45.45％,无EBV感染组ERK蛋白表达阳性率为16.00％,差异有统计学意义(x2=4.854,P<0.05).两组肝功能指标差异均无统计学意义.结论 EBV感染的肝硬化患者外周血清单核细胞CD14++CD16-亚群、CD14++CD16+亚群及NK细胞中Tim-3、PD-1表达升高,ERK蛋白表达升高,但EBV感染对患者肝硬化肝功能指标无明显影响.

abstractsObjective To investigate the effects of Epstein-Barr virus (EBV) infection on T cell immunoglobulin- and mucin-domain-containing molecule-3 (Tim-3), programmed cell death protein 1 (PD-1) and extracellular regulated protein kinases (ERK) in liver cirrhosis patients. Methods In this study, 47 patients with hepatitis B cirrhosis were selected from August, 2017 to August, 2018 as the research subjects and were divided into two groups of EBV infection group and non-EBV infection group according to EBV infection. Expressions of serum Tim-3, PD-1 and ERK in the two groups were compared. Results The differences of the expressions of PD-1 and Tim-3 in CD14++CD16- and CD14++CD16+ peripheral blood monocyte subsets, and in NK cells of the two groups were statistically significant (P<0.05). The positive rate of ERK expression in EBV infection group was 45.45％ while the rate in non-EBV infection group was 16.00％. The difference was statistically significant (P<0.05). There was no statistically significant difference in liver function indexes between the two groups (P>0.05). Conclusions Elevated expression of Tim-3, PD-1 in CD14++CD16- and CD14++CD16+ peripheral blood monocyte subsets and in NK cells, as well as increased ERK expression were observed in EBV infected liver cirrhosis patients. EBV infection showed no significant effect on liver functions in patients with liver cirrhosis.