摘要目的 评价胶体金技术检测甲型/乙型流感病毒的有效性.方法 选取我院231例儿童患者的口咽拭子采集物,应用荧光PCR技术进行甲型/乙型流感病毒核酸的定性检测,同时用胶体金技术进行甲型/乙型流感病毒的抗原定性检测.评价胶体金技术与荧光PCR技术比较的一致性、灵敏度、特异度.结果 与荧光PCR技术比较,胶体金技术检测甲型/乙型流感病毒的一致性较好,Kappa值分别为0.895、0.925、0.900.通过荧光PCR技术检测出甲型流感病毒阳性74例,乙型流感病毒阳性33例;胶体金技术检测出甲型流感病毒阳性75例,乙型流感病毒阳性30例.与荧光PCR技术比较,在甲型流感病毒检测中胶体金技术的灵敏度为95.95％,特异度为96.75％,差异无统计学意义(P=1.000);在乙型流感病毒检测中胶体金技术的灵敏度为87.88％,特异度为99.17％,差异无统计学意义(P=0.375);在甲型/乙型流感病毒检测中胶体金技术的灵敏度为93.46％,特异度为95.97％,差异无统计学意义(P=0.774).结论 在甲型/乙型流感病毒的检测中,胶体金技术与荧光PCR技术一致性较好,同时具有良好的灵敏度与特异度.

abstractsObjective To evaluate the effectiveness of using immune colloidal gold technique in the detection of influenza A/B viruses. Methods Throat swab samples from 231 child patients in our hospital were collected. Fluorescent PCR and immune colloidal gold assays were used for qualitative detection of the nucleic acids and antigens of influenza A /B viruses, respectively. The consistence , specificity and sensitivity of immune colloidal gold assays comparing to fluorescent PCR were evaluated. Results The immune colloidal gold assay showed good consistence with fluorescent PCR in detection of influenza A and B viruses, with Kappa values of 0.895, 0.924 and 0.900, respectively. By PCR, 74 cases were influenza A virus positive and 33 cases were influenza B virus positive. The immune colloidal gold assay detected 75 cases of influenza A virus and 30 cases of influenza B virus. Comparing to the PCR method, the sensitivity and the specificity of immune colloidal gold assay in detection of influenza A were 95.95％ and 96.75％, respectively. There was no statistically significant difference (P=1.000). The sensitivity and the specificity of immune colloidal gold assay in detection of influenza B virus were 87.88％and 99.17％, respectively. There was no statistically significant difference (P=0.375). The sensitivity and the specificity of immune colloidal gold assay in detection of influenza A/B viruses were 93.46％ and 95.97％, respectively. There was no statistically significant difference (P=0.774). Conclusions The immune colloidal gold assay showed good consistence with fluorescent PCR in detection of influenza A/B viruses and had good sensitivity and specificity.