摘要目的:了解南昌市生活污水中非脊灰肠道病毒型别。方法:从2018年1月至2020年12月，在南昌市某污水处理厂每月采集生活污水样本，用RD和Hep-2细胞对阴离子膜吸附－碱洗脱法富集的病毒进行分离。通过反转录-聚合酶链式反应（RT-PCR）对收获的毒株核酸进行扩增，并对获得的VP1区扩增片段进行序列分析。结果:2018—2020年共采集生活污水36份，通过RD、Hep-2两种细胞共分离非脊灰肠道病毒（non-polio enterovirus，NPEV）207株，包含8种不同血清型，均属于HEV-B组。南昌市生活污水中常见非脊灰肠道病毒的优势基因型为ECHO 11、CoxB 3、ECHO 6和ECHO 7，且ECHO 11、ECHO 6和ECHO 7在环境污水和AFP病例中存在共循环现象。结论:2018—2020年南昌市生活污水NPEV检出率较高，需持续开展外环境NPEV监测以评估NPEV相关疾病的风险。

abstractsObjective:To understand the types of non-poliovirus enteroviruses (NPEV) from domestic sewage in Nanchang city.Methods:The domestic sewage samples were collected from a sewage processing plant in Nanchang during January 2018 to December 2020. RD and Hep-2 cell culture were used in virus isolation for samples enriched by the anionic membrane adsorption -alkali washing method. The reverse transcription polymerase chain reaction (RT-PCR) was used to amplify nucleic acid from harvested virus strains and the amplified VP1 region were used for sequence analysis.Results:A total of 36 domestic sewage samples were collected from 2018 to 2020, and 207 NPEV strains were isolated from RD and Hep-2 cells. The strains belonged to 8 serotypes in the HEV-B group. The common dominant genotypes were ECHO 11, CoxB 3, ECHO 6 and ECHO 7 in the domestic sewage of Nanchang city while ECHO 11, ECHO 6 and ECHO 7 were co-circulating in environmental sewage and AFP cases.Conclusions:The detection rate of NPEV in domestic sewage in Nanchang was high from 2018 to 2020. Continuous environmental surveillance for NPEV would be necessary to assess the risk of NPEV-related diseases.