摘要目的:探索BRAF V600E突变比值（The Ratio of BRAF V600E expression），在预测甲状腺乳头状癌（papillary thyroid carcinoma，PTC）颈部淋巴结转移（lymph node metastasis，LNM）中的临床价值。 方法:收集于浙江大学医学院附属杭州市第一人民医院2018年1月至2019年1月间手术治疗的234例PTC患者，采用微滴数字PCR（droplet digital PCR，ddPCR）定量法检测病理石蜡标本BRAF V600E突变比值，分析突变比值与PTC患者LNM的相关性，构建受试者工作特征曲线（ROC）。 结果:PTC患者伴有LNM者的BRAF V600E突变比值显著高于无LNM者（ P<0.01），ROC曲线显示BRAF V600E突变比值预测LNM的敏感性、特异性、阳性预测值、阴性预测值和曲线下面积（AUC）分别为27.7%、91.0%、73.3%、58.5%、0.581。 结论:利用PTC病理石蜡标本中BRAF V600E突变比值预测LNM有较高的特异性，可为PTC颈淋巴结清扫提供临床决策。

abstractsObjective:To investigate the clinical value of the ratio of BRAF V600E expression for predicting lymph node metastasis(LNM) in papillary thyroid carcinoma (PTC). Methods:From January 2018 to January 2019, 234 patients underwent surgery in our hosptial and were enrolled in this study. The ratio of BRAF V600E expression was quantified by droplet digital PCR(ddPCR) in formalin-fixed paraffin-embedded tissues(FFPE) to analyze the correlation with LNM. The receiver operating characteristic curve (ROC) was constructed to evaluate the diagnostic value of the ratio of BRAF V600E expression for LNM. Results:The ratio of BRAF V600E expression with LNM is significantly higher than that of without LNM ( P<0.01). The ROC show the sensitivity, specificity, positive predictive value, negative predictive value and area under the curve (AUC) are 27.7%, 91.0%, 73.3%, 58.5% and 0.581, respectively. Conclusion:The ratio of BRAF V600E expression in FFPE is highly specific for predicting the diagnosis of LNM in PTC, which may help to provide clinical decisions for cervical lymph node dissection.