摘要目的 探讨6 Gy137Csγ射线一次性全身照射后,小鼠血清中白细胞介素10(IL-10)、白细胞介素6(IL-6)和肿瘤坏死因子α(TNF-o)水平的变化及对脂多糖刺激反应性的远期影响.方法 将实验小鼠分为假照射组和照射组,假照射组不接受照射,照射组给予6Gyγ射线照射.照射后10周,将假照射组和照射组小鼠按组别分别提前24 h和1h腹腔注射脂多糖(20 mg/kg),对照组注射生理盐水(100μl/只).取小鼠外周血,利用酶联免疫吸附技术检测小鼠血清中IL-10、IL-6和TNF-t的水平.结果 假照射组小鼠在给予脂多糖刺激1h后,血清中IL-10、IL-6和TNF-α水平与其对照组相比均显著升高(t=7.31、2.71和15.09,P分别为＜0.01、＜0.05和＜0.01).照射组小鼠在给予脂多糖刺激1h后,血清中IL-10、IL-6和TNF-α水平与其对照组相比均显著升高(t=4.14、7.18和5.14,P均＜0.01).与假照射组相比,照射组小鼠在给予脂多糖刺激24h后,TNF-α和IL-6水平无明显变化,IL-10水平升高了19.9％(t=2.84,P＜0.05).结论 经6Gyγ射线照射后10周,小鼠免疫调节功能尚未完全恢复;在接受脂多糖刺激后,假照射组和照射组小鼠的抗感染能力也有差异.脂多糖对辐射后小鼠血清中IL-10、IL-6和TNF-α水平的影响及其意义仍需进一步研究.

abstractsObjective To investigate the effects of lipopolysaccharide (LPS) on interleukin-10(IL10),interleukin-6(IL-6) and tumor necrosis factor-α(TNF-α) levels in mice exposed whole body to 6 Gy γ-ray radiation once.Methods The mice were divided into sham-irradiation group and irradiation group.Mice in sham-irradiation group accepted no irradiation,while mice in irradiation group accepted 6 Gy whole body irradiation at dose rate of 0.8 Gy/min.Mice in LPS treated group accepted intraperitoneal injection of LPS (20 mg/kg) at 10 weeks after irradiation,while mice in control group were accepted intraperitoneal injection of saline.The peripheral blood was collected by eyeballs enucleation after 1 h and 24 h respectively,then IL10,IL-6 and TNF-α levels were detected by enzyme-linked immunoassay.Results Compared to control group,the levels of IL-10,IL-6 and TNF-α in blood serum increased at 1 h and 24 h after LPS injection both in sham-irradiation group (t=7.31,P＜0.01; t=2.71,P＜0.05; t=15.09,P＜0.01 ) and irradiation group(t＝4.14,7.18 and 5.14,all P＜0.01 ).Compared to sham-irradiation group,the level of IL-10 increased 19.9％ at 24 h after LPS injection in irradiation group(t=2.84,P＜0.05),while the levels of TNF-α and IL-6 had no significant differences between sham-irradiation group and irradiation group.Conclusions The immune system of mice had not entirely recovered at 10 weeks after 6 Gy y-ray irradiation.After LPS injection,there was difference of anti-infection ability between sham-irradiation group and irradiation group.The effects of LPS on cytokines IL-10,IL-6 and TNF-α levels in radiation-exposed mice need to be further explored.