摘要目的 了解不同剂量γ射线照射对雌果蝇产生的辐射损伤与氧化效应.方法 采用不同剂量137Cs γ射线照射白眼W.18、红眼UAS-EGFP和Actin-Gal4雌果蝇,再测定其生存、运动和生殖能力.通过测定雌果蝇体内过氧化氢酶(CAT)活力和丙二醛(MDA)含量对果蝇成虫的辐射和氧化损伤进行评价.组间比较均采用LSD-t检验进行统计学分析.结果 137Csγ射线对W1118、UAS-EGFP、Actin-Gal4的半数致死剂量分别为1513、1643、1809 Gy;3个品系3日龄雌果蝇成虫的生存率、攀爬高度分数、产卵量与γ射线照射剂量呈负相关;与未照射组相比,1550 Gy照射后雌果蝇成虫体内的CAT活力降低,差异有统计学意义(t=10.76、13.84、11.22,均P＜0.05);MDA含量升高,差异有统计学意义(t=4.51、4.26、4.35,均P＜0.05).结论 高剂量γ射线照射导致的雌果蝇成虫的生存、运动和生殖能力降低可能与其氧化损伤相关.

abstractsObjective To investigate the radiation damage and oxidative effects of different doses by gamma radiation on female adult Drosophila.Methods Three strains of female Drosophila melanogaster of white eye W1118,red eye UAS-EGFP,and Actin-Gal4 were treated with different doses of 137Cs-gamma rays.Then,their survival,movement,and reproductive abilities were measured.The oxidative damage of the female adult Drodophila.melanogaster was evaluated by measuring catalase (CAT) activity and malondialdehyde (MDA) content.Results The half lethal doses of W1118,UAS-EGFP,and Actin-Gal4 were 1513,1643,and 1809 Gy,respectively.The survival rate,climbing height fraction,and fecundity of the three-day-old female Drosophila were negatively correlated with the radiation dose.After irradiation,the activities of CAT decreased (t=10.76,13.84,11.22,all P＜0.05) and the content of MDA increased (t=4.51,4.26,4.35,all P＜0.05) in the female Drosophila adult body.Conclusion The reduced survival,movement,and fecundity of female adult Drosophila.melanogaster may be related to the oxidative damage under high doses of gamma radiation.