摘要目的 在体外建立诱导结核分枝杆菌耐异烟肼药物产生的全过程,分析结核分枝杆菌产生耐药的机理.方法 对结核分枝杆菌强毒株(H37RV)进行诱导试验,与30株临床耐异烟肼药物分离株共同做聚合酶链反应-单链构象多态性(PCR-SSCP)和序列分析.结果 在第7代H37RV的PCR-SSCP电泳出现异常,经测序证实发生katG基因突变,与部分临床突变株的类型基本相同.结论 用药物间断性地不断刺激结核分枝杆菌,是产生结核分枝杆菌耐异娴肼药物的重要原因之一.

abstractsObjective To preliminarily analyze the mechanism of drug resistance tuberculosis by observing the entire process of isoniazid-resistant tuberculosis in vitro experiments.Methods The tuberculosis virulent strain (H37RV) induced in the different levels of drug and 30 isolated strains of clinical drug resistance were detected by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and sequence analysis.Results PCR-SSCP electrophoresis was abnormal in 7th generation of H37RV.Confirmed by sequencing,katG gene occurred mutation,same as clinical mutant type.Conclusions Stimulating constantly tubercle bacillus with drug is an important reason that causes isoniazid-resistance.