摘要目的 观察4-羟基壬烯醛(4-HNE)对气道上皮细胞基质金属蛋白酶2(MMP-2)、基质金属蛋白酶9(MMP-9)和环氧合酶2(COX-2)表达的影响.方法 Western-blot和RT-PCR检测不同浓度4-HNE(0μmol/L,10 μmol/L,30 μmol/L和50 μmol/L)作用气道上皮细胞4h后MMP-2、MMP-9和COX-2蛋白和mRNA表达的变化.结果 4-HNE以剂量依赖的方式促进MMP-2、MMP-9、COX-2mRNA表达和蛋白合成,与对照组相比,差异具有统计学意义(P＜0.05).结论 4-HNE可通过上调气道上皮细胞COX-2的表达而导致慢性阻塞性肺疾病患者慢性气道炎症的发生、发展,上调MMP-2、MMP-9的表达引起慢性阻塞性肺疾病患者的气道重塑.

abstractsObjective To observe the influence of 4-hydroxy-2-nonenal (4-HNE) on expression of MMP-2,MMP-9 and COX-2.Methods Airway epithelial cells were incubated with 4-HNE of different concentrations (0 μmol/L,10 μmol/L,30 μmol/L and 50 μmol/L) for 4 hours.The protein and mRNA expression of MMP-2,MMP-9 and COX-2 were detected by using Western-blot and RT-PCR.Results 4-HNE could promote MMP-2,MMP-9,COX-2 mRNA expression and protein synthesis in a dose dependent manner.Compared with the control group,the difference was significant (P ＜ 0.05).Conclusions 4-HNE could lead to chronic airway inflammation and airway remodeling in COPD patients by upregulating COX-2,MMP-2 and MMP-9 expression of airway epithelial cells.