摘要目的 探讨急性喉气管支气管炎患儿血清IL-4、γ干扰素(IFN-γ)和IgE水平的动态变化及临床意义.方法 采用ELISA双抗体夹心法检测22例急性喉气管支气管炎患儿入院时(急性期)、出院前(临床症状消失)及正常对照组儿童血清IL-4、IFN-γ水平,采用荧光酶联免疫法检测IgE水平,并进行比较.结果 ①急性喉气管支气管炎患儿血清IL-4水平入院时显著高于对照组(P＜0.05),出院时较入院时明显下降(P＜0.05),与对照组比较差异无统计学意义(P＞0.05);②急性喉气管支气管炎患儿血清IFN-γ水平入院时显著低于对照组(P＜0.05),出院时较入院时明显升高(P＜0.05),与对照组比较差异无统计学意义(P＞0.05);③急性喉气管支气管炎患儿血清IgE水平入院时显著高于对照组(P＜0.05),出院时较入院时明显下降(P＜0.05),与对照组比较差异无统计学意义(P＞0.05).结论 急性喉气管支气管炎患儿存在免疫功能紊乱,IL-4、IFN-γ和IgE在患儿免疫病理机制中起重要作用.

abstractsObjective To investigate clinical significance and the change of serum IL-4,IFN-γ and IgE levels in infant with acute laryngotracheobronchitis.Methods Serum samples were obtained from 22 children with acute laryngotracheobronchitis,and 25 healthy children.The change of IL-4,IFN-γ and IgE levels in serum when hospital admission (acute stage)and before discharge (the clinical symptom vanished)were observed.ELISA was used to determine the levels of IL-4,IFN-γ in serum.Serum IgE level was determined with enzyme-linked fluoroimmuneassay.Results ①The serum level of IL-4 in patients with acute laryngotracheobronchitis when hospital admission were much higher than that in control group (P ＜0.05).It was decreased before discharge and significantly lower than that when hospital admission (P ＜0.05).There was no different between that in patients before discharge and that in control group (P ＞ 0.05).②The serum level of IFN-γ in patients with acute laryngotracheobronchitis when hospital admission were much lower than that in control group (P ＜0.05).It was increased before discharge and significantly higher than that when hospital admission (P ＜0.05).There was no different between that in patients before discharge and that in control group (P ＞0.05).③The serum level of IgE in patients with acute laryngotracheobronchitis when hospital admission were much higher than that in control group (P ＜ 0.05).It was decreased before discharge and significantly lower than that when hospital admission (P ＜ 0.05).There was no different between that in patients before discharge and that in control group (P ＞ 0.05).Conclusions There is immunologic disorder in infants with acute laryngotracheo-bronchitis.IL-4,IFN-γ and IgE play important role in immunologic pathogenesis.