摘要目的：观察红霉素对4-羟基壬烯醛(4-HNE)刺激的气道上皮细胞基质金属蛋白酶2(MMP-2)、MMP-9、环氧合酶2(COX-2)和活化蛋白-2(AP-2)表达的影响。方法 Western blot 检测红霉素(5μmol/L)预孵育不同时间(24、36、48 h)后4-HNE(10μmol/L 作用4 h)刺激的气道上皮细胞MMP-2、MMP-9、COX-2和 AP-2蛋白表达的变化。结果红霉素预孵育36 h 和48 h 后明显抑制4-HNE刺激的气道上皮细胞 MMP-2、MMP-9、COX-2合成，明显抑制4-HNE 刺激的气道上皮细胞核转录因子 AP-2的激活，与红霉素未预孵育组相比，差异具有统计学意义(P <0.05)。结论红霉素可明显抑制4-HNE 刺激的气道上皮细胞 MMP-2、MMP-9、COX-2表达，其机制可能和抑制核转录因子 AP-2的激活有关。

abstractsObjective To observe the effects of erythromycin on the expression of matrix metalloproteinase-2 (MMP-2 ),MMP-9,cyclooxygenase-2 (COX-2 )and activator protein-2 (AP-2 )in airway epithelial cells induced by 4-hydroxy-2-nonenal (4-HNE).Methods Airway epithelial cells were preincubated with 5 μmol/L erythromycin for different time (24,36,48 hours )and incubated with 10 μmol/L 4-HNE for four hours.The protein expressions of MMP-2,MMP-9,COX-2,and AP-2 were detected by Western blot.Results Preincubation with 5 μmol/L erythromycin for 36 hours and 48 hours significantly inhibited the expressions of MMP-2,MMP-9,COX-2,and AP-2 in airway epithelial cells induced by 4-HNE.Compared with the control group,the difference was significant (P < 0.05 ). Conclusions Erythromycin could inhibit the expressions of MMP-2,MMP-9,and COX-2 in airway epithelial cells induced by 4-HNE,which may be associated with inhibiting the stimulation of AP-2.