摘要目的：探讨香烟烟雾提取物(cigarette smoke extract,CSE)诱导脐静脉内皮细胞内质网应激(endoplasmic reticulum stress,ERS)对中性粒细胞趋化作用的影响。方法实验分4组：正常对照组、CSE刺激组、ERS抑制剂组和阴性对照组。培养人脐静脉内皮细胞(human umbilical vein endothelial cell,HUVECs),给予不同浓度的 CSE 刺激24 h,Real time-PCR 法检测糖调节蛋白78(glucose regulated protein 78,GRP78)、趋化因子8(CXCL-8)、细胞间黏附分子1(intercellular adhesion molecule-1, ICAM-1)、E-selectin mRNA 的表达水平；用 ERS 抑制剂4-苯基丁酸(4-phenylbutyrate,4-PBA)干预,ELISA法检测各组 HUVECs 的 CXCL-8蛋白表达水平,Transwell趋化实验检测各组培养上清对正常人外周血中性粒细胞的趋化作用。结果 CSE 增强 GRP78及CXCL-8、ICAM-1、E-selectin mRNA的表达,并在一定范围内具有浓度依赖性；ERS 抑制剂4-PBA减少CXCL-8的分泌(q=8.370,P<0.05),并减弱中性粒细胞的趋化作用(q=4.808,P<0.05)。结论 CSE诱导 HUVECs ERS,并上调 CXCL-8、ICAM-1、E-selectin的基因转录；4-PBA 减弱中性粒细胞的趋化作用,可能与减少CXCL-8分泌作用有关。

abstractsObjective To investigate the effect of endoplasmic reticulum stress (ERS) induced by cigarette smoke extract (CSE) in neutrophil migration.Methods Human umbilical vein endothelial cell (HUVECs) were cultured and divided into 4 groups:normal control group,CSE-stimulated group,CSE-stimulated with 4-PBA groups,CSE-stimulated with negative control groups.HUVECs were cultured and stimulated with CSE at concentrations(0%,5%,10% and 20%)respectively for 24 h,mRNA of CXCL-8,ICAM-1,E-selectin and GRP78 were detected by Real-time PCR.ELISA was performed to test the expression of CXCL-8 protein,and neutrophil migration was detected by Transwell board test.Results The mRNA of CXCL-8,ICAM-1,E-selectin and GRP78 increased in CSE-stimulated HUVECs (P <0.05),furthermore,it was concentration-dependent.4-PBA significantly reduced the expression of CXCL-8 protein (P <0.05) and neutrophil migration (P <0.05).Conclusions Cigarette smoke extract induces ER stress in HUVECs,which may play a part in increasing the expressions of CXCL-8,ICAM-1 and E-selectin.The reduction of neutrophil migration may relate to the lower expression of CXCL-8.