摘要目的 探讨非类固醇类抗炎药NS398对肺癌H460细胞增殖及其Kazal基序逆向诱导半胱氨酸丰富蛋白(RECK)及膜型基质金属蛋白酶1(MT1-MMP)表达的影响.方法 应用MTT方法检测H460细胞生长的抑制率,用免疫荧光法、Western blot法检测RECK及MT1-MMP蛋白的表达.结果 NS398可抑制H460细胞的增殖,促进RECK蛋白的表达,减少MT1-MMP蛋白的含量,并呈剂量依赖关系.结论 NS398可通过促进肺癌细胞RECK的表达并减少MT1-MMP的含量,进而抑制肺癌H460细胞的生长,这可能是肺癌侵袭转移的一个重要机制.

abstractsObjective To explore the effect of NS398 on the expressions of reversion-inducingcysteine-rich protein with kazal motifs (RECK) and membrane type 1-matrix metalloproteinase (MT1-MMP) in human H460 lung carcinoma cells.Methods The H460 cell growth inhibition rate was detected by MTT assay.Immunofluorescence assay and Western blot were used to analyse the expressions of RECK and MT1-MMP protein levels in H460 cells.Results NS398 inhibited the proliferation of H460 cells,promoted the expression of RECK protein,reduced the expression of MT1-MMP protein in a dosedependent manner.Conclusions NS398 may increase the expression of RECK and decrease the expression of MT1-MMP in lung carcinoma cells,which can inhibit the growth of H460 cells.It is probably a key mechanism of NS398 regulating invasion and migration of lung carcinoma cells.