摘要目的 补体成分1q亚单位结合蛋白(C1QBP)参与多种恶性肿瘤的发生和发展,本研究拟评估C1QBP和非小细胞肺癌(NSCLC)的关系.方法 选取西安交通大学第二附属医院NSCLC标本共46例及正常肺组织24例,通过免疫组织化学法检测C1QBP表达水平,分析患者TNM分期、淋巴结转移、细胞分化程度和C1QBP表达的关系,并对C1 QBP蛋白进行生物信息学分析.结果 肺腺癌和肺鳞癌中C1QBP蛋白的表达明显高于癌旁正常肺组织(P＜0.05);C1QBP阳性表达的强弱与患者性别、年龄、TNM分期、淋巴结转移、病理类型无关,与肿瘤的分化程度有关.生物信息学分析提示C1QBP蛋白主要定位于线粒体,具有MAM33结构域,在信号转导、应激反应和免疫反应等方面发挥一定的作用;STRING数据库筛选的激肽原1和C1QBP有相互作用.结论 C1QBP可能和NSCLC的发生有关,有望成为新的诊断标志物和治疗靶点.

abstractsObjective It has been proven that complement component 1q subcomponent binding protein (C1QBP) might play an important role in the oncogenesis and progression of numerous malignant tumors.The aim of this study is to evaluate the relationship between C1QBP and non-small cell lung cancer.Methods The expression of C1QBP was detected by immunohistochemical method in 46 cases of non-small cell lung cancer and 24 cases of normal lung tissue in the Second Affiliated Hospital of Xi'an Jiaotong University.TNM stage,lymph node metastasis,cell differentiation and expression of C1QBP were evaluated.The bioinformatic of C1QBP were analyzed.Results The expression of C1QBP was significantly higher in NSCLC tissues than that in normal lung tissues (P ＜0.05).The strength of positive expression was not correlated with the gender,age,TNM stages,lymph node metastasis and histological type,and was correlated with the degree of tumor differentiation.Bioinformatic analysis suggested that C1QBP was mainly located in the mitochondria and has MAM33 domain.C1QBP plays a certain role in signal transduction,stress response and immune response.The KNG1 protein screened by the STRING database interacts with C1QBP.Conclusions C1QBP may play an important role in the oncogenesis of NSCLC.C1QBP may be a novel biomarker for the diagnosis of NSCLC and a new target for its treatment.