摘要目的 了解杭州地区2003-2007年从爆发和散发细菌性痢疾患者中分离之福氏志贺菌4c亚型(F4c)菌株的分子生物学特征.方法 对24株F4c菌株以生化反应和血清分型进行鉴定,利用PCR法检测侵袭性质粒抗原H基因(ipaH)及脉冲场凝胶电泳(PFGE)进行分型分析.结果 24株菌株全部为F4c,均具有ipaH毒力基因.用PFGE分型方法将24株菌株分成17种不同的带型,Dice系数为0.71～1.00.2005年、2006年、2003年三次爆发(分别简称为爆发1、2、3)中各自分离到的菌株基因型紧密相关.三次爆发中的部分菌株可能存在一定的相关性(Dice系数>0.8).散发菌株和爆发1、3菌株间的距离较远(Dice系数<0.8),和爆发2菌株间可能存在一定的相关性(Dice系数>0.8).结论 F4c菌株具有ipaH毒力基因,PFGE提示杭州地区近年F4c三起爆发菌株可能属共同的克隆群.

abstractsObjective To analysis the molecular biological characteristics of Shigella flexneri 4c isolates from patients of shigellosis outbreak and sporadic diaxrbea in Hangzhou during 2003-2007. Method 24 S. flexneri 4c isolates were identified by biochemical tests and serotyping. Among these isolates, invasive plasmid antigen H gene (ipaH) was examined by PCR, and molecular typing was performed by Pulse-field gel electrophoresis(PFGE). Results All 24 isolates were serotyped as S. flexneri 4c and were positive for ipaH gene. A total of 17 different PFGE patterns were identified among these 24 isolates with Dice coefficient varying from 0.71 to 1.00. On the basis of PFGE patterns, S. flexneri 4c isolates from each of three shigellesis outbreaks were closely related. Some isolates from three outbreaks (outbreak in 2005,2006 and 2003 year which named as outbreak 1,2,3 respectively) were possibly related (Dice coefficient > 0.8). The sporadic isolates were demonstrated to be distinct from the isolates from two outbreaks (outbreak 1,3) ( Dice coefficient <0.8). However, the sporadic isolates were possibly related to isolates from outbreak 2 (Dice coefficient > 0.8). Conclusions All S. flexneri 4c in Hangzhou possess ipaH. The S. flexneri 4c isolates from three recent outbreaks in Hangzhou may belong to the same clone.