换一批摘要传统的病原体检测方法在检测敏感性、特异性、应用范围等方面存在许多不足.PCR技术已被用于感染早期的病原体检测,但在应用过程中也面临不少问题.近年来出现的新的核酸扩增技术可弥补普通PCR技术的缺点.此文就其中依赖核酸序列的扩增技术、PCR-胶体金免疫层析技术和多重荧光实时PcR技术进行综述.
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abstractsThe traditional pathogen detection mathods have many disadvantages in the sensitivity, specificity and application range. There are still some problems in PCB, which has been used for pathogen detection in the early stage of infection. New nucleic acid amplification technologies may make up the deficiency of PCR. In this review, three methods including nucleic acid sequence-based amplification (NASBA), PCR-immunochromatography test and multiplex fluorescent real-time PCR technology are described.
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关键词
核酸扩增技术病原体检测PCR-免疫层析技术多重荧光实时PCRNucleic acid amplification techniquesPathogen detectionPCR-Immunochromatography testMultiplex fluorescent real-time PCR
分类号
R4（临床医学）
栏目名称
DOI
10.3760/cma.j.issn.1673-4149.2009.01.022
发布时间
2009-04-21（万方平台首次上网日期，不代表论文的发表时间）
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