摘要目的:了解我国HIV CRF01_AE亚型及其相关重组亚型的包膜糖蛋白(envelope glycoprotein，ENV)分子流行情况，建立ENV共识序列以了解氨基酸突变及蛋白质三级结构。方法:收集Los Alamos数据库中2015—2021年我国HIV-1 CRF01_AE亚型及其相关重组亚型的完整ENV基因序列，构建系统发育进化树并建立ENV共识序列，使用Protscale及PROVEAN对ENV共识序列广谱中和抗体表位中的氨基酸亲疏水性变化及有害性突变进行预测，同时分析氨基酸突变对ENV三级结构的影响。结果:系统发育分析结果显示，有70.0%（14/20）的CRF01_AE亚型序列属于进化簇4，在ENV共识序列的广谱中和抗体表位V1/V2及Loop D中分别存在4个和1个的氨基酸突变使亲疏水性发生变化，PROVEAN预测结果显示V1/V2位点的G152D及β23/V5/β24表位的M476I为有害突变。ENV共识序列的蛋白质三级结构pLDDT值为86.2，在V1/V2表位存在由于有害突变所产生的新α螺旋。结论:我国HIV-1 CRF01_AE亚型基因谱系众多，加剧了重组病毒的形成。在ENV共识序列V1/V2结构域中由于氨基酸突变使其蛋白空间结构发生改变，这种改变可能会对后续广谱中和抗体的诱导产生影响。

abstractsObjective:To understand the molecular epidemiology of HIV CRF01_AE subtype and its related recombinant subtype envelope glycoprotein (ENV) in China, and to establish ENV consensus sequences to analyze amino acid mutations and protein tertiary structure.Methods:Full-length ENV gene sequences of HIV-1 CRF01_AE subtype and related recombinant subtypes in China from 2015 to 2021 were collected from the Los Alamos database. A phylogenetic evolutionary tree was constructed, and ENV consensus sequences were established. Protscale and PROVEAN were used to predict changes in amino acid hydrophobicity in broad neutralizing antibody epitopes of the ENV consensus sequence and harmful mutations, and the impact of amino acid mutations on the tertiary structure of the ENV protein was analyzed.Results:Phylogenetic analysis showed that 70.0%(14/20) of CRF01_AE subtype sequences belonged to evolutionary cluster 4. In the broad neutralizing antibody epitope V1/V2 and Loop D of the ENV consensus sequence, there were 4 and 1 amino acid mutations, respectively, causing changes in hydrophobicity. PROVEAN predicted that the mutations G152D at the V1/V2 and M476I at the β23/V5/β24 epitopes were harmful. The protein tertiary structure pLDDT value of the ENV consensus sequence was 86.2, with a new α helices generated in the V1/V2 epitope due to harmful mutations.Conclusions:The genetic lineage of HIV-1 CRF01_AE subtype in China is diverse, contributing to the formation of recombinant viruses. Amino acid mutations in the V1/V2 structural domain of the ENV consensus sequence result in changes in its protein spatial structure, which may impact the subsequent induction of broad neutralizing antibodies.