摘要目的 通过多西紫杉醇处理前列腺癌细胞,探讨多西紫杉醇治疗雄激素抵抗前列腺癌可能的分子机制.方法 利用三种前列腺癌细胞系LNCaP、PC-3和CW22-rv1作为研究对象,用多西紫杉醇处理后,蛋白质印迹和细胞免疫荧光检测p-c-Jun的表达差异并检测其存活率.结果 p-c-Jun的表达结果显示,PC-3细胞最高,LNCaP细胞次之,CW22-rv1最低；PC-3细胞的存活率最低,LNCaP细胞次之,CW22 - rv1最高.结论 多西紫杉醇对不同的前列腺癌细胞株的作用效果不同.多西紫杉醇可能通过JNK细胞信号通路影响p-c-Jun的表达而调控细胞生长.p-c-Jun蛋白的表达水平与细胞生存率呈负相关,p-c-Jun表达水平与细胞对DTX的敏感性呈负相关.

abstractsObjectives This study was conducted to assess the molecular mechanism of docetaxel treatment for castration - resistant prostate cancer.Methods The p - c - Jun expression was tested by Western blotting in PC - 3,CW22 - rv1 and LNCaP cell lines.Cell survival was also detected in the above cell lines after exposing to Docetaxel.Results The expression of p - c - Jun was increased significantly,but cell survival was the lowest in the PC - 3 cell.The expression of p - c - Jun was the lowest in CW22 - rv1 cell and the cell survival was the most.The expression of p - c - Jun and the cell survival in LNCaP were in the middle of PC - 3 and CW22 - rv1 cell lines.Conclusions Different effects of docetaxel treatment for prostate cancer cell lines was different.Docetaxel may induce the expression of p - c - Jun through JNK cell signaling pathways,and the level of p - c - Jun was negatively coupled with the cell survival.Thus,the increased expression of p - c - Jun are somehow associated with the decreased sensitivity of prostate cancer to DTX.