摘要目的 分析不同结核病患者体内CD4+CD25+Foxp3+调节性T细胞(Tr)表达的变化,探讨其在结核病免疫中的作用.方法 对33例结核病患者以及同期30例健康体检者运用流式细胞术检测其外周血CD4+CD25high和CD4+CD25+Foxp3+Tr表达情况.结果 结核组CD4+CD25high和CD4+CD25+Foxp3+Tr检测结果分别为(8.84±2.55)%、(6.30±1.38)%,高于对照组(7.09±1.09)%、(5.22±0.64)%,差别有统计学意义(t=3.57,4.01,P＜0.01);痰涂阳患者CD4+CD25high和CD4+CD25+Foxp3+Tr检测结果分别为(10.52±3.27)%、(7.18±1.77)%,高于痰涂阴患者(8.21±1.94)%、(5.97±1.06)%,两组间差别均具有统计学意义(t=2.51,2.42,P＜0.05);初治患者和复治患者间CD4+CD25high和CD4+CD25+Foxp3+检测结果无统计学意义(t=0.03,0.02,P＞0.05).结论 结核病患者体内CD4+CD25high和CD4+CD25+Foxp3+Tr检测结果高于健康人群,提示患者体内存在免疫系统抑制状态.

abstractsObjective To investigate the expression of CD4 + CD25 + Foxp3 + regulatory T cell in patients with tuberculosis, and to discover its role in the immune response to mycobacterium tuberculosis. Methods Thirty-three patients with tuberculosis and 30 healthy controls were selected who were consulted in our hospital. Patients were classified by their chemotherapy and smear sputum and CD4 + CD25 + Foxp3 + regulatory T cell were detected by flow cytometry. Results Expression of CD4 + CD25high and CD4+ CD25 + Foxp3 + regulatory T cell in experimental group were ( 8.84 ± 2.55 ) % and (6.30 ± 1.38 ) % respectively, which were significantly higher than they were in control group (t = 3.57,4. 01, P ＜ 0. 01 ). The expression of CD4 + CD25high and CD4+ CD25 + Foxp3 + regulatory T cell in patients with positive smear sputum were also significant higher than that in patients with negative smear sputum ( t = 2. 51,2. 42,P ＜ 0.05). No significance was founded between the first-visit group and revisit group ( t = 0. 03, 0. 02, P ＞ 0.05 ). Conclusions CD4 + CD25high and CD4 + CD25 + Foxp3 + regulatory T cell in patients with tuberculosis were higher than healthy control, which may result in immune suppression.