摘要目的观察对叶百部碱对Toll样受体2(TLR2)和MyD88的调节作用，以探寻其抗结核的可能作用机制。方法 取对叶百部碱单体作用于感染结核杆菌的U937单核巨噬细胞，分别采用RT-QPCR、Western Blot和FACS方法检测用药前后TLR2和MyD88的表达差异。结果 实验结果显示对叶百部碱具有上调TLR2和MyD88的作用。结论对叶百部碱抗结核作用可能通过调节TLR2-MyD88信号通路而发挥作用。

abstractsObjective Explore the potential molecular mechanism of Tuberostemonine against tuberculosis infection. Methodes Analysis and compare the different expression of TLR2 and MyD88 in U937 cells infected with Mycobacterium tuberculosis(Mtb)strain H37Ra before and after treatment with Tuberostemoninel, by realtime PCR, FACS and western blot. Results The mRNA and protein expression of TLR2 and MyD88 in U937 cells could be upregualted by Tuberostemonine, which reduced when infected with H37Ra.Conclusion Tuberostemonine might exert the effect against tuberculosis infection by regulating the TLR2-MyD88 pathway through improving the expression of TLR2 and MyD88 in macrophages infected with Mtb.