摘要目的 评估人类白细胞抗原G(HLA-G)和人类白细胞抗原E(HLA-E)的表达在宫颈癌进展中的意义及对患者预后的影响.方法 采用聚合酶链反应和流式细胞术检测正常组、CIN Ⅰ、CINⅡ、CINⅢ(宫颈上皮内瘤变Ⅰ-Ⅲ级)及浸润癌组HLA-G和HLA-E基因及蛋白的表达,比较两者在各组表达的差异及其对病人长期生存的影响.结果 CINⅢ及浸润癌组HLA-G表达与CIN Ⅰ、CINⅡ和正常组比较U值分别为564.0、536.0和565.5,P均＜0.05,HLA-E前两组的表达与后者比较U值分别为550.0、542.0和578.5,P均＜0.05;并且HLA-G和HLA-E阳性的患者生存率明显低于阴性者(P＜0.05).结论 HLA-G和HLA-E的异常表达促使宫颈癌的发生和发展,并且影响患者的术后长期生存.

abstractsObjective To evalute the clinical significance and effect on prognosis of human leukocyte antigen G and E in cervical cancer.Methods To detect the expression of HLA-G and HLA-E gene and protein from the normal group,CIN Ⅰ,CIN Ⅱ,CIN Ⅲ (cervical intraepithelial neoplasia Ⅰ,Ⅱ and Ⅲ) and invasive carcinoma group using PCR-SSP and flow cytometry to compare the different expression of both in each group and determine its effect on patients' long-term survival.Results U values of HLA-G in CIN Ⅲ and invasive cancer group compared with CIN Ⅰ,CIN Ⅱ and normal group was 564.0,536.0 and 565.5 respectively (P ＜0.05),The Values of HLA-E that was 550.0,542.0 and 578.5 respectively (P ＜ 0.05);and long-term survival rate of HLA-G and HLA-E positive patients was significantly worse than the negative cohort (P ＜ 0.05).Conclusion The aberrant expression of HLA-G and HLA-E promotes the occurrence and development of cervical cancer,in addition it can affect the patient's long-term survival rate.