摘要目的 研究上皮性卵巢癌中E盒结合锌指蛋白2(ZEB2)和转化生长因子β1(TGF-β1)的表达及预后意义.方法 采用免疫组化SP法检测52例上皮性卵巢癌、23例卵巢良性上皮性肿瘤和15例正常卵巢组织中ZEB2和TGF-β1的表达,分析二者在卵巢上皮癌中的相关性及与临床病理特征和预后的关系.结果 ZEB2和TGF-β1在上述三种组织中均定位于细胞质,其阳性表达率分别为67.3％、21.7％、13.3％和71.2％、30.4％、20.0％,差异均有统计意义(x2=21.34、18.087,p均＜0.05);ZEB2和TGF-β1在卵巢上皮癌中的表达存在正相关(rs=0.552,P＜0.05),且与分化程度、FIGO分期有关(P＜0.05),与年龄、病理类型无关(P＞0.05);ZEB2和TGF-β1表达与卵巢上皮癌患者总生存率有关(P＜0.05);COX风险比例模型显示ZEB2阳性表达是预后危险因素(OR=2.653,P＜0.05).结论ZEB2和TGF-β1可能促进上皮性卵巢癌进展,ZEB2可能作为评估预后的新指标.

abstractsObjective To investigate the expression and prognosis significance of zinc-finger E-box binding homeobox 2 (ZEB2) and transforming growth factor β1 (TGF-β1) in epithelial ovarian cancer.Methods Immunohistochemistry SP method was adopted to detect the expression of ZEB2 and TGF-β1 in 52 specimens of epithelial ovarian cancer,23 cases of benign ovarian tumor,and 15 cases of normal ovarian tissue.In patients with epithelial ovarian cancer,the relationship among the expression of ZEB2,the expression of TGF-β1,the clinical and pathological characteristics and the prognosis were then evaluated.Results ZEB2 and TGF-β1 in epithelial ovarian cancer,benign epithelial ovarian tumor and normal ovarian tissue were both located in cytoplasm,and the positive expression rate of ZEB2 and TGF-β1 were 67.3％,21.7％,13.3％ (x2 =21.340,P ＜0.05) and 71.2％,30.4％,20.0％ (x2 =18.087,P ＜ 0.05),respectively.In epithelial ovarian cancer,positive correlation between the expression of ZEB2 and TGF-β1 was observed(r =0.552,P ＜ 0.05).The expression of ZEB2 and TGF-β1 was correlated with tumor differentiation degree and FIGO stage(P ＜ 0.05),but no correlation with age and pathological type was observed (P ＞ 0.05).The overall survival rate of epithelial ovarian cancer patients was related to the expression of ZEB2 and TGF-β1 (P ＜ 0.05).Multivariate COX regression revealed that the positive expression of ZEB2 was a risk factor for the prognosis of epithelial ovarian cancer(OR =2.653,P ＜ 0.05).Conclusion ZEB2 and TGF-β1 proteins may promote the development of epithelial ovarian cancer,and ZEB2 may be a new indicator to predict the prognosis.