摘要目的:分析免疫性血小板减少症(imumune thrombocytopenia，ITP)儿童外周血中miRNA-150以及血小板膜糖蛋白(glycoprotein，Gp)特异性抗体表达情况和临床意义。方法:选择2020年1月至2022年12月济宁医学院附属医院儿科收治的ITP患儿总共98例设为观察组，另选择同时间济宁医学院附属医院儿科健康体检儿童100例设为对照组，采用实时荧光定量反应检测并比较两组研究对象外周血miRNA-150以及Gp特异性抗体水平，分析观察组不同疾病分期和不同疗效患儿外周血中miRNA-150水平及有关抗体表达情况。结果:观察组患儿外周血miRNA-150水平高于对照组[(1.14±0.30)比(0.39±0.05)， t＝24.65， P<0.05]。观察组中新发ITP组的miRNA-150水平、抗GPIIb/Ⅲa抗体阳性率高于持续性ITP组以及慢性ITP组[(1.24±0.22)比(1.15±0.20)、(0.88±0.16)，75.00%比54.05%、61.90%， F/ χ2值为14.35、11.38， P值均<0.05]。观察组中治疗后显效56例，有效21例，无效21例。显效组的miRNA-150水平、抗GPIIb/Ⅲa抗体阳性率均低于有效组以及无效组(0.66±0.14)比(0.98±0.23)、(1.20±0.35)，12.50%比47.62%、80.95%， F/ χ2值为10.68、6.50， P值均<0.05)，抗GPIIb/Ⅲa、抗GPIb/Ⅸ均为阴性的比例高于有效组以及无效组[83.93%比(38.10%、4.76%)， P<0.05]。 结论:ITP儿童外周血中miRNA-150呈高表达状态，且新发患者miRNA-150、抗GPIIb/Ⅲa抗体阳性率更高，miRNA-150以及在Gp特异性抗体在评估ITP患儿疾病分期以及疗效中具有重要价值。

abstractsObjective:To analyze the expression and clinical value of miRNA-150 and platelet membrane glycoprotein(Gp) specific antibodies in peripheral blood of children with immune thrombocytopenia(ITP).Methods:A total of 98 children with ITP admitted to the affiliated hospital of jining medical college from January 2020 to December 2022 were selected as the observation group, and a total of 100 healthy children undergoing physical examination in our hospital during the same period were selected as the control group.The levels of miRNA-150 and Gp specific antibodies in peripheral blood were detected, and the levels of miRNA-150 in peripheral blood of the two groups were compared.The levels of miRNA-150 and related antibodies in peripheral blood of children with different disease stages and different therapeutic effects in the observation group were compared.Results:The level of miRNA-150 in the peripheral blood of the observation group was higher than that of the control group[(1.14±0.30) vs (0.39±0.05), t=24.65, P<0.05]. In the observation group, the miRNA-150 level and the positive rate of anti GPIIb/Ⅲa antibody in the newly diagnosed ITP group were higher than those in the persistent ITP group and the chronic ITP group[(1.24±0.22)vs(1.15±0.20), (0.88±0.16), 75.00%vs 54.05%, 61.90%, F / χ2 values were 14.35 and 11.38, both P values <0.05]. In the observation group, there were 56 cases of complete reactions after treatment, 21 cases of effective reactions, and 21 cases of ineffective reactions.The miRNA-150 level and positive rate of anti GPIIb/Ⅲa antibodies in the complete response group were lower than those in the effective and ineffective groups(0.66±0.14)vs(0.98±0.23), (1.20±0.35), 12.50% vs 47.62%, 80.95%, F / χ2 values were 10.68 and 6.50, both P values<0.05), the positive rates of negative anti GPIIb/Ⅲa and anti GPIb/Ⅸ were higher than those of the effective and ineffective groups[83.93% vs (38.10%, 4.76%), P<0.05]. Conclusion:The expression of miRNA-150 in the peripheral blood of children with ITP is high, and the positive rates of miRNA-150 and anti GPIIb/Ⅲa antibodies in newly diagnosed patients are higher. miRNA-150 and Gp-specific antibodies have important value in evaluating disease staging and efficacy in ITP children.