摘要目的 探讨可卡因-苯丙胺调节转录肽(cocaine- and amphetamine-regulated transcript peptides,CART)对缺血再灌注小鼠梗死体积和脑组织4-羟壬烯醛(4-hydroxy-2 -noneral,HNE)含量的影响.方法 健康雄性小鼠随机分为缺血再灌注组(n=27)、CART组(n=27)、生理盐水对照组(n=27)和假手术组(n=15).建立大脑中动脉闭塞(middle cerebral artery occlusion,MCAO)模型,CART组和生理盐水对照组在MCAO2 h后分别经尾静脉注射给予CART55-102和同体积生理盐水,然后每隔24 h重复1次.在再灌注后12、24、48和72 h分别进行神经功能评分、梗死体积和脂质氧化应激代谢产物HNE含量检测.结果 CART可显著改善脑缺血再灌注后不同时间点神经功能缺损评分(P均＜0.05),显著缩小梗死体积(P均＜0.05)；再灌注后各时间点HNE含量均显著增高(P均＜0.05),CART可显著下调缺血后脑内HNE水平升高(P均＜0.05).结论 CART能通过抑制脂质过氧化保护小鼠缺血性脑损伤.

abstractsObjective To investigate the effect of cocaine-amphetamine-regulated transcript peptide (CART) on the content of 4-hydroxy-2-noneral (HNE) and infarct volume after cerebral ischemia/reperfusion in mice.Methods A total of 96 healthy male mice were randomly divided into four groups:ischemia/reperfusion (n =27),CART (n =27),normal saline control (n =27) and sham operation (n =15) groups.A middle cerebral artery occlusion (MCAO) model was induced.Two hours after MCAO,CART 55-102 and equivalent normal saline were injected respectively via the tail veins of mice in the CART group and the normal saline control group,and then they were injected every other 24 hour.The neurological scores,infarct volume and the HNE content of lipid metabolism of oxidative stress were performed and detected respectively at 12,24,48 and 72hours after reperfusion.Results CART could significantly improve the neurological deficit scores (all P ＜0.05) and reduce infarct volume (all P＜0.05) at different time points after ischemia/reperfusion.The content of HNE was upregulated (all P＜0.05) at different points after referfusion.CART could significantly down-regulate the increased HNE levd in brain after ischemia (all P＜0.05).Conclusions CART may protect ischemic brain injury in mice by inhibiting lipid peroxidation.