摘要目的 研究注射临床低剂量和高剂量的丙泊酚对大鼠空间关联记忆能力的影响.方法 将30只大鼠随机分成3组:对照组、临床低剂量0.1 mg/(kg·min)组和临床高剂量0.5 mg/(kg·min)组.在注射丙泊酚1 d后进行水迷宫实验,包括4 d的定位航行和1 d的空间探索实验.在定位航行实验中分别对3组大鼠进行Ⅰ、Ⅱ和Ⅲ象限入水的训练,并记录3组大鼠的逃避潜伏期、游泳路程和游泳速度.在空间探索实验巾将大鼠从未训练的Ⅳ象限入水,分别记录平台象限游泳时间、第一环点数以及总得分.结果 在第1,2天的定位航行实验中,低剂量组与对照组大鼠的逃避潜伏期没有统计学差异(P>0.05);高剂量组逃避潜伏期比对照组显著延长(P<0.05).第3,4天的定位航行实验中,3组大鼠之间均未见差异(P>0.05).在空间探索实验中,从未训练的Ⅳ象限入水时,对照组、低剂量组和高剂量组大鼠的总得分分别为(2 509.36±190.72)、(2 378.55±210.69)和(1 954.85±174.20).低剂量组和对照组没有显著性差异(P>0.05);高剂量组与对照组存在显著性差异(P<0.05),即高剂量组比对照组总得分低.结论 注射低剂量的丙泊酚不影响大鼠的空间关联记忆和空间记忆能力;高剂量丙泊酚在注射后前2 d内降低大鼠的空间记忆能力,但2 d后对大鼠的空间记忆能力没有影响,说明高剂量的丙泊酚降低了大鼠的空间关联记忆能力.

abstractsObjective To study the clinical effects of low-dose and high-dose injection of propefol on spatial associative memory capacity in rats. Methods 30 rats were randomly divided into 3 groups: control, clin-ical low-dose propofol (0.1 mg/kg·min) group and clinical high-dose propofol (0.5 mg/kg·min) group. The experi-ments including 4-days place swimming and 1-day space exploration began from the day after injection. In place swimming group, rats were trained in morris water maze from Ⅰ,Ⅱ,Ⅲ quadrant. Latency period, swimming length and swimming velocity were recorded. In space exploration group, untrained rats were put into the maze from the Ⅳ quadrant and swimming time, first circling point value and total scores were recorded. Results At the first two days of place swimming test there were no differences between the control group and the low-dose group in la-tency, swimming length and swimming velocity(P<0.05); while high-dose group's latency period and swimming length were obviously longer than that of control(P<0.05). At the second two days there were no differences among the three groups(P<0.05). In the space exploration test, total scores of control, low-dose group and high-dose group were: 2 509.36±190.72, 2 378.55±210.69 and 1 954.85±174.20, respectively. There was no difference be-tween low-dose group and the control group(P<0.05). Total scores of the high-dose group were much lower than that of control. Conclusion The clinical low-dose propofol injection has no effects on the spatial associative memory capacity and spatial memory capacity of rats. The clinical high-dose propofol injection affects the rats' spatial memory capacity during the first two days but has no effects after two days, while the clinical high-dose propofol injection reduces the rats' spatial associative memory capacity.