摘要目的:检测复制因子C4（RFC4）在胰腺导管腺癌组织中的表达情况，初步探究RFC4与胰腺导管腺癌的临床预后关系。探讨RFC4作为胰腺导管腺癌潜在的生物标志物的可能性。方法:通过生物信息学的方法分析RFC4在胰腺导管腺癌组织及癌旁正常组织中的mRNA水平，并分析其表达与胰腺导管腺癌患者的生存率间的关系。回顾性分析76例接受手术治疗的胰腺导管腺癌患者的临床病理资料。采用免疫组化法检测胰腺导管腺癌组织和癌旁正常组织中的RFC4蛋白的表达水平，分析其与胰腺导管腺癌患者临床病理特征的关系。结果:生物信息学分析结果显示，RFC4的mRNA在胰腺导管腺癌组织中显著高表达，并与患者总生存率（ P=0.046）与无病生存率（ P=0.042）显著相关。免疫组化结果表明，RFC4在胰腺导管腺癌组织中的表达明显高于癌旁正常组织。RFC4在胰腺导管腺癌组织中的高表达与肿瘤大小相关（ P=0.043），而与年龄、性别、肿瘤分级的相关性无统计学意义（均 P>0.05）。 结论:胰腺导管腺癌组织中RFC4呈显著高表达并提示不良预后，且其表达水平与胰腺导管腺癌患者肿瘤大小有关。RFC4可能作为胰腺导管腺癌潜在的预后预测因子。

abstractsObjective:To detect the expression of replication factor C4(RFC4) in pancreatic ductal adeno-carcinoma, and to explore the clinical prognosis of RFC4 and pancreatic ductal adenocarcinoma. To explore the possibility of RFC4 as a potential biomarker for pancreatic ductal adenocarcinoma.Methods:The mRNA level of RFC4 in pancreatic ductal adenocarcinoma and normal tissues adjacent to the cancer was analyzed by bioinformatics methods, and the relationship between its expression and the survival rate of patients with pancreatic ductal adenocarcinoma was analyzed. The clinicopathological data of 76 patients with pancreatic ductal adenocarcinoma who underwent surgical treatment were retrospectively analyzed. Immunohistochemical method was used to detect the expression level of RFC4 protein in pancreatic ductal adenocarcinoma tissue and normal tissues adjacent to the cancer, and to analyze its relationship with clinicopathological characteristics of patients with pancreatic ductal adenocarcinoma.Results:The results of bioinformatics analysis showed that RFC4 mRNA was significantly highly expressed in pancreatic ductal adenocarcinoma tissue, and was significantly correlated with the overall survival rate ( P=0.046) and disease-free survival rate ( P=0.042) of the patients. Immunohistochemical results showed that the expression of RFC4 in pancreatic ductal adenocarcinoma tissue was significantly higher than that in normal tissues adjacent to the cancer. The high expression of RFC4 in pancreatic ductal adenocarcinoma tissue was related to tumor size ( P=0.043), but not related to age, gender, and tumor grade (all P>0.05). Conclusions:RFC4 is highly expressed in pancreatic ductal adenocarcinoma tissues and indicates a poor prognosis, and its expression level is related to the tumor stage of pancreatic ductal adenocarcinoma. RFC4 may serve as a new prognostic predictor for pancreatic ductal adenocarcinoma.