摘要目的 探讨中国汉族人群不同个体红细胞M、N血型抗原数量的表达情况.方法 从962例中国汉族献血人群中随机选取MM型、MN型、NN型各50例,以血型血清学、PCR-SSP基因分型技术确定血型,应用流式细胞术分析M抗原和N抗原表达量.结果 962例献血者中MM型占24.01%、MN型占47.19%、NN型占28.79%.选取的150例样本基因分型与血清学结果完全相符.MM型个体间M抗原平均荧光强度极差为432.78;MN型个体间M抗原平均荧光强度极差为425.06;MN型个体间N抗原平均荧光强度极差为6.38;NN型个体间N抗原平均荧光强度极差为35.74.MM型的M抗原数量约为MN型的2.686倍;NN型的N抗原数量约为MN型的4.268倍.结论 中国汉族人群M或N抗原数量的表达在不同个体间差异明显.

abstractsObjective To explore individual expression difference of the M and N antigens on the red blood cell in Chinese Han population. Methods Fifty cases with MM,MN and NN phenotypes were chosen from 962 subjects of Chinese Han population, which have been identified by serology. The genotypes of the total 150 cases were confirmed by PCR-SSP, and the quantities of M and N antigens were determined by flow cytometry. Results In 962 Chinese Han individuals, the phenotype frequencies of MM, MN and NN were 24.01 %, 47.19 % and 28.79 %, respectively. The genotyping results of selected 150 samples detected by PCR-SSP were completely in accordance with the results detected by serology. The mean fluorescence intensity (MFI) of the M antigen was 432.78 on MM individuals and 425.06 on MN individuals. The M antigen in MM group was 2. 686 times the amount of which in MN group. On the other hand, MFI of the N antigen was 6.38 in NN group and 35.74 in MN group. The N antigen in NN group was 4. 268 times the amount of which in MN group. Conclusion There is a significant difference of expression amount of the M or N antigens among different blood groups in Chinese Han population.