摘要目的 研究乳腺癌患者外周血中人乳珠蛋白(hMAM-mRNA)的表达与乳腺癌微转移的关系.方法 以MCF-7乳腺癌细胞为阳性对照,利用Nested-RT-PCR技术检测92例乳腺癌术后患者、20例乳腺良性肿瘤患者、10例其他系统恶性肿瘤患者、10例正常人群外周血hMAM-mRNA表达情况.结果 hMAM-mRNA仅在乳腺癌患者外周血中表达.92例乳腺癌患者外周血中hMAM-mRNA表达率为43.5%(40/92),hMAM-mRNA在外周血中的表达与ER、PR状态,患者月经状况,肿瘤大小无关.与HER-2、CEA、CA15-3在外周血中是否阳性表达及患者年龄相关.与腋窝淋巴结转移状况密切相关.结论 外周血中hMAM-mRNA检测是乳腺癌血微转移可供参考的临床指标.

abstractsObjective To study the correlation between micrometastasis and human manmmaglobin mRNA(hMAM-mRNA)in tlle peripheral blood of the patients with breast cancer.Methods Peripheral blood samples were obtained from 92 patients with breast carcinoma,and 20 patients were with benign breast diseases,and 10 patients with other system malignant tumor,and 10 normal healthy volunteers.To identify breast carcinoma cells in periphersl blood,hMAM-mRNA Was amplified from total RNA extracted from whole blood by nested reverse transcription-polymerase chain reaction(Nested-RT-PCR).The expression of hMAM-mRNA wag contracted to MCF-7 breast cancer cell.Results The hMAM-mRNA wag only detectable in the blood from breast disease patients.Among 92 breast carcinoma patients,40 were nested-RT-PCR positive for hMAM-mRNA.The positive rate was 43.5%(40/92).The expression of hMAM-mRNA in pe-ripheral blood was not correlated with patient's estrogen or pregnant receptor;menstruation state;primarytumour size.The expression of hMAM-mRNA in peripheral blood wag correlated with patient's age:the state of c-erbB-2 and carcino embryonic antigen and carbohydrate antigen 15-3.Conclusion The hMAM-mRNA in peripheral blood may be indicator of the micmmetastasis of breast cancer.