摘要目的 建立一种新型引物系统,通过PCR的方法来检测B和C型乙型肝炎病毒.方法 通过在引物3′末端第6个碱基位置通过引入3个脱氧腺苷(AAA或TTT)改良引物系统,结合Taqman荧光探针PCR建立一种检测乙肝病毒分型的系统.并用该方法检测108个乙肝病毒阳性的血清标本.结果 以C型乙肝病毒为例,此系统可检测出1000 IU/mL的模板,混合模板的检测能力达到0.1％.108例标本,36例(33.3％)标本为B型,70例(64.8％)为C型,2例(1.9％)为B和C混合感染,我们的结果与测序结果一致.结论 此种新型引物结合荧光定量PCR的方法可以检测出血清中微量B和C型乙型肝炎病毒以及二者混合型基因型,是一种高效、经济、可靠,可用于检测乙肝病毒B和C型基因型的方法.

abstractsObjective The objective of this study was to develop an accurate novel primer sys-tem assay for detecting genotypes B and C of HBV .Methods We established novel primers through in-serting 3 nt deoxidized-poly nucleotide ( AAA or TTT ) into normal primer at 3′-segment n-6 position , Taqman probe was used as signal system .Results The novel primer system could specifically detect a limit of 1000 IU/mL target template , and measure 0 .1％ target genotype in a mixed population .We test 108 samples by novel primer system , and found 36 ( 33 .3％) samples of them were infected with genotype B , 70 ( 64 .8％) samples infected with genotype C and 2 ( 1 .9％) samples infected with mixed genotypes .The results were almost complete consistency with those by directly sequencing .Conclusion The novel primer system was an efficient , reliable , and cost effective method for chronic HBV infection genotyping .