摘要目的:观察IGF2蛋白对小鼠早胚体外发育的影响。方法:①体外培养：取野生型和 IMP2基因敲除组小鼠受精卵，分别在含或不含IGF2的M16培养基中进行体外连续培养，观察各组发育至囊胚的数目和囊胚发育率；②胚胎移植：分别将4组早胚移植回代孕雌鼠，观察4组代孕雌鼠的产仔率和妊娠率。 结果:在体外培养实验中，对比无IGF2的囊胚形成率(29.4%)，野生型添加IGF2组的胚胎发育到囊胚率明显提高(65.8%)，差异具有统计学意义( P＝0.002)。 IMP2基因敲除鼠无IGF2组和IGF2组几乎都不能发育到囊胚，差异无统计学意义( P＝0.060)。胚胎移植实验中，接受IGF2处理过的野生型胚胎的代孕母鼠平均每个雌性产下更多的幼崽，并且怀孕率也明显高于接受未处理过的对照胚胎的雌性，差异具有统计学意义( P<0.001)。 结论:IGF2可显著提高野生型小鼠囊胚形成率和胚胎发育能力，但对 IMP2基因敲除小鼠胚胎无影响。

abstractsObjective:To observe the effect of IGF2 on mouse early embryos in vitro development.Methods:① In vitro culture: Take fertilized eggs from mice in the control group and IMP2 knockout group, culture them continuously in M16 medium with or without IGF2, and then observe and calculate number and rate of blastocyst development in each group; ② Embryo transfer: Transferring the four groups early embryos back to surrogate female mice, and observing the birth rate and pregnancy rate of the four surrogate female mouse groups. Results:In vitro culture, the blastocyst formation rate was 29.4% without IGF2 and 65.8% with IGF2 ( P=0.002). The IMP2 knockout groups rarely developed to blastocysts( P=0.060). In embryo transfer experiment, the surrogate mothers that received IGF2 embryos gave birth to more pups per female on average, and the pregnancy rate was significantly higher than that of control group( P<0.001). Conclusion:IGF2 significantly increased blastocyst formation rate and embryonic development ability in control group, but had no effect on IMP2 gene knockout mice.