摘要目的 建立一种原子吸收分光光度法,定量检测山梨醇中的镍含量,并确认该方法的可行性.方法 采用标准加入法,在4份等量山梨醇样品中分别加入3 μg/ml镍标准母液0.0、0.5、1.0、1.5 ml.经10 g/L吡咯烷二硫代氨基甲酸铵溶液和甲基异丁基甲酮处理后,取萃取液,测定其在232.0 nm处的吸光度.结果 用标准加入法得到的标准曲线线性良好,决定系数＞0.998.镍加样回收率为98％～103％,测定结果的相对标准偏差为3％.该法测定镍的定量限为质量分数4.6×10-7.结论 建立的原子吸收分光光度法可以用于山梨醇中镍含量的定量检测.

abstractsObjective To establish an atomic absorption spectrophotometric method for quantitative detection of nickel content in sorbitol,and confirm the feasibility and validity of the method.Methods Standard addition technique was used.In the 4 aliquots of sorbitol sample,0.0,0.5,1.0 and 1.5 ml of 3 μg/ml nickel stock standard solution was added respectively.Then,10 g/L ammonium pyrrolidine dithiocarbamate solution and methyl isobutyl ketone (MIBK) solution were added for extraction of nickel into MIBK layer.The liquid MIBK layer containing nickel was analyzed at 232.0 nm.Results The coefficient of determination was ＞0.998,which indicated the linearity of the method was excellent.The recovery of nickel was determined as 98％-103％,and the maximum relative standard deviation was 3％,which indicated that the accuracy and repeatability of the method were quite good.Also the limit of quantitation was determined as 4.6 × 10-7.Conclusion The established atomic absorption spectrophotometric method can be used for determining nickel content in sorbitol.