摘要目的:建立快速、简便和准确地检测表达绿色荧光蛋白的重组呼吸道合胞病毒（recombinant respiratory syncytial virus expressing the enhanced green fluorescent protein，RSV-EGFP）感染滴度的方法。方法:以反向遗传学构建的RSV-EGFP为研究对象、流式细胞术为检测方法，建立以泊松分布为基础的数学模型并做曲线拟合，以数学模型中的预设参数进行病毒感染滴度计算。结果:成功建立了基于泊松分布的病毒感染数学模型和流式细胞术检测RSV-EGFP感染滴度的新方法。与传统的荧光显微镜计数法比较，检测结果差异无统计学意义（ t=0.16， P=0.879）；6次测定结果的变异系数为10.42%（荧光显微镜计数法为60.44%），重复性好。 结论:建立了适用于RSV-EGFP感染滴度测定的新方法，可为其他病毒感染滴度的测定方法研究提供参考。

abstractsObjective:To establish a rapid, simple, and accurate method for detecting the infection titer of recombinant respiratory syncytial virus expressing the enhanced green fluorescent protein (RSV-EGFP).Methods:Utilizing an RSV-EGFP generated through reverse genetics as the subject and employing flow cytometry as the detection method, a mathematical model was developed based on the Poisson distribution for curve fitting. With this model, the viral infection titer was determined using pre-established parameters.Results:A new method for detecting RSV-EGFP infection titer by mathematical model of virus infection based on Poisson distribution and flow cytometry was successfully established. Compared with the traditional fluorescence microscope counting method, detection results of the new method had no statistically significant difference ( t=0.16, P=0.879).The coefficient of variation of 6 experiments was 10.42% for the new method, while was 60.44% for fluorescence microscope counting method, showing good repeatability. Conclusion:A new method suitable for the determination of RSV-EGFP infection titer is established, which can also provide a reference for the determination of other virus infection titers.