摘要目的 探讨芪竹方粗提物对MGC-803细胞血管内皮生长因子(VEGF)及其血管内皮生长因子受体(KDR/ilk-1)蛋白表达的影响.方法 MGC-803细胞处理方法被分成空白对照、二甲基亚砜(DMSO)对照、芪竹方粗提物高(500 μg/ml)、低浓度(250 μg/ml)四组,采用流式细胞仪、western bloting 两种方法测定VEGF、KDR/flk-1蛋白的表达量.结果 空白对照、DMSO对照、芪竹方粗提物高、低浓度四组在流式细胞术检测中VEGF表达量分别是120.0±10.8、116.8±14.7、95.0±12.5、108.4±13.5,与空白对照组比较,芪竹方粗提物高浓度组VEGF表达降低,具有显著性差异:KDR/flk-1分别是10.4±3.5、9.0±3.4、6.8±2.3、6.8±3.5,各组间差异无统计学意义.在western bloting法检测中VEGF蛋白表达量灰度值分别是14.45±4.61、12.32±3.27、2.58±0.84、3.45±1.12,芪竹方粗提物高浓度、低浓度组均明显低于空白对照组,差异具有显著性;KDR/flk-1蛋白表达量灰度值分别是3.87±1.05、3.55±1.32、3.62±1.01、3.73±0.88,各组间差异无统计学意义.结论 芪竹方粗提物对MGC-803细胞VEGF表达有下调作用,对KDR/flk-1表达无明显影响.

abstractsObjective To evaluate the influence Qizhu decoction on VEGF and its receptor KDR/flk-1 protein expression in MGC803 cell. Methods Four groups of MGC-803 Cells were established, and intervened with blank control, DMSO control, high dose Qizhu decoction, and low dose Qizhu decoction respectively. VEGF and KDR/flk-1 protein were detected by flow cytometry and western bloting. Results The value of VEGF expression was 120.0±10.8, 116.8±14.7, 95.0±12.5, and 108.4±13.5 respectively in each group. The difference between high dosage Qizhu decoction group and the bland control group was significant. Value of KDR/flk-1 were 10.4±3.5, 9.0±3.4, 6.8±2.3, and 6.8±3.5 in each group respectively. There was no significant difference among these 4 groups. The grayseale values of VEGF expression was 14.45±4.61, 12.32±3.27, 2.58±0.84, and 3.45±1.12 in each group respectively. There was significant difference between the QiZhu groups and the blank control group. Meanwhile grayseale values of KDR/flk-1 expression were 3.87±1.05, 3.55±1.32, 3.62±1.01, and 3.73±0.88 in each group respectively, showing no significant difference among 4 groups. Conclusion Rough extraction of QiZhu decoction down-regulated the protein expression of VEGF, but had no effects on the expression of KDR/flk-1.