摘要目的 探讨瓜蒌薤白类方(自拟复方蒌薤合剂)对实验性动脉粥样硬化(atherosclerosis,AS) 大鼠血管反应的影响.方法 采用高脂饲料喂养Wister 大鼠制备动脉粥样硬化模型.大鼠按体重进行随机区组设计,分为4 组各10 只,正常对照组(A 组)、高脂模型组(B 组)、高脂阿托伐他汀治疗组(C 组,灌服阿托伐他汀10 mg/kg·d-1,qd)和高脂复方蒌薤合剂治疗组(D 组,灌服复方蒌薤合剂bid),共饲养干预10 周.①在0 周和10 周末取大鼠血浆,测定内皮素(ET-1 )、一氧化氮(NO)和一氧化氮合酶(NOS); ②10 周末取大鼠胸主动脉制备血管环,测定血管环对不同浓度乙酰胆碱(Ach)及硝普钠(SNP)的舒张反应性,以量-效曲线、最大舒张反应(Emax )及引起最大舒张反应一半时的药物浓度(IC50)表示血管反应性.结果 ① B 组0 周与10 周时血浆NO、NOS 水平明显降低[NO 0 周时为(35.73±3.72)μmol/L,10 周时为(19.03±1.72)μmol/L;NOS 0 周时为(24.78±0.25)U/ml,10 周时为(15.36±0.24)U/ml,P 均＜0.01];ET-1 水平明显上升[0 周时为(34.58±4.00)pg/ml,10 周时为(117.58±5.34)pg/ml,P＜0.01].阿托伐他汀和复方蒌薤合剂均可提高血浆NO 和NOS 水平[NO C 组:(31.30±1.96)μmol/L;D 组:(32.85± 3.70)μmol/L),P＜0.01];[NOS C 组:(21.96±1.07)U/ml;D 组:(19.78±1.20)U/ml,P＜0.01]; 降低ET-1 水平[C 组:(58.26±5.14)pg/ml;D 组:(59.30±5.73)pg/ml,P＜0.01];②各组大鼠对SNP 诱导的非内皮依赖性舒张反应差异无统计学意义[EmaxA 组:(97.33±1.31)%;B 组:(98.24±1.04)%; C 组:(97.52±1.09)%;D 组:(97.91±1.59)%,P 均＞0.05];对Ach 诱导的内皮依赖性舒张反应差异有统计学意义[EmaxA 组:(72.65±3.31)%;B 组:(32.68±2.39)%;C 组:(61.63±2.07)%;D 组:(57.58± 2.43)%,P 均＜0.01],B 组较A 组量效曲线非平行左移,C 组、D 组较B 组可使量效曲线非平行右移,C、D 两组组间比较差异无统计学意义[Emax C 组:(61.63±2.07)%;D 组:(57.58±2.43)%,P＞0.05].结论 中药复方蒌薤合剂对动脉粥样硬化后血管舒张功能下降有保护作用.

abstractsObjective To investigate the effect of Chinese medicine Louxie mixture on vascular reactivity in atherosclerosis rats. Methods Forty male rats were randomly divided into four groups: the control group (A group, n＝10) was fed with normal diet; the model group (B group, n＝10), atorvastatin treated group (C group, n＝10 and Louxie mixture group (D group, n＝10) were fed with high fat/cholesterol diet. Atorvastatin 10 mg/kg·d-1 was administered to C group and Louxie mixture to D group for 10 weeks by gavages. Serum endothelin-1 (ET-1), nitric oxide (NO) and nitric oxide syntheses (NOS) were observed in different groups before and after the treatment. Vascular reactivity of aortic rings was measured by both the sodium nitroprusside(SNP)-induced endothelium-independent relaxation (NEDR) and the acetylcholine (Ach)-induced endothelium-dependent relaxation (EDR) in different groups. Results After treatment, the (19.03±1.72)μmol/l; NOS (24.78±0.25)U/ml vs (15.36±0.24U/ml), P＜0.01], while the level of ET-1in B levels of NO and NOS in B group were significantly lower than those in A group [NO(35.73±3.72)μmol/l vs group was higher than that in A group [(34.58±4.00) pg/ml vs (117.58±5.34)pg/ml,P＜0.01]. The levels of NO and NOS were significantly increased and the level of ET-1 was decreased in C and D groups after the treatment [NO(C: 31.30±1.96 umol/l;D: 32.85±3.70 umol/l); NOS (C: 21.96±1.07 U/ml ; D: 19.78± 1.20U/ml ); ET (C:58.26±5.14 pg/ml; D:59.30±5.73 pg/ml), P＜0.01]. The activities of NEDR were similar in four groups[SNP Emax (A: 97.33±1.31; B: 98.24±1.04;C: 97.52±1.09; D: 97.91±1.59)%, P＞0.05], but the level of EDR in the B group (P＜0.01) was the lowest among four groups [Ach Emax (A: 72.65±3.31; B: 32.68±2.39;C: 61.63±2.07; D: 57.58±2.43)%, P＜0.01]. Conclusion Chinese medicine Louxie mixture can protect vascular function in atherosclerosis rats.