摘要目的 观察同病异证H22肝癌小鼠甲状腺AC-cAMP信号通路基因差异表达的特征.方法 采用小鼠计量化四诊和辨证方法及GeneChip Mouse Exon 1.0 ST Array等技术,观察肿瘤邪毒证、气虚证甲状腺激素生成和释放相关AC-cAMP信号通路及相关基因表达特征.结果 ①一些基因相对关键,Tg和转录因子Pax8等在肿瘤发生早期已下调,TSHr在邪毒证下调、气虚证略上调.②多数基因表现为气虚证表达量略大于邪毒证,包括甲状腺主要标志性基因Nis和Tpo、腺苷酸环化酶AC、转录因子Ttf1和Ttf2,PKA催化亚基Prkaca等;③其他基因在气虚证、邪毒证互有高低.结论 同病异证H22肝癌小鼠甲状腺AC-cAMP途径,TSHr、AC、Pax8、Ttfl、Titf2、Tg、Nis、Tpo等重要基因转录特征具有一致性,提示邪毒壅盛证者甲状腺功能处于抑制状态.

abstractsObjective To observe gene transcription characteristic of AC-cAMP signal pathway in thyroid of H22 tumor-bearing mice with different syndromes.Methods The quantitative four diagnosis and syndrome differentiation methods and Affymetrix Gene Chip Mouse Exon 1.0 ST Array were used,thyroid gene expression of normal mice,qi-deficiency syndromes (QDS) and poisonous pathogenic factors syndrome (PPFS) in early stage of H22 tumor-bearing mice were detected.Genes of AC-cAMP signal pathway in gene chips,which related with thyroid hormone synthesis and secretion were selected,and then analyzed their expressive characteristic.Results ①In key genes,TG and Pax8 were down-regulated in early stage,TSHr down-regulated in PPFS while contrary in QDS.②the transcription level of most genes in QDS were slightly higher than in PPFS,Nis,Tpo,AC,Ttf1,Titf2 and Prkaca were included.()Slight changes showed in other genes in this pathway.Conclusion In AC-cAMP pathway of H22 tumor-bearing mice with different symptoms,some key genes showed similar characteristics including TSHr,AC,Pax8,Ttf1,Tiff2,TG,Nis and Tpo.This suggested that the thyroid is inhibited in mice with PPFS.