摘要目的 探讨不同浓度藤黄酸(gambogic acid,GA)对结肠癌LoVo细胞株增殖、侵袭能力及基质金属蛋白酶-2 (MMP-2)蛋白表达的影响.方法 CCK-8法检测藤黄酸对LoVo细胞增殖的影响;Transwell小室检验LoVo细胞体外侵袭能力的改变;Western Blot检测LoVo细胞中MMP-2蛋白的改变.结果 CCK-8检测显示,GA剂量1、2、4、6、8mol/L作用于细胞24h、48h后,对结肠癌Lovo细胞增殖的抑制率分别为(o.16±0.11)％、(0.24±0.08)％、(0.58±0.01)％、(0.67±0.03)％、(0.79±0.01)％和(0.27±0.05)％、(0.69±0.09)％、(0.85±0.01)％、(0.87±0.01)％、(0.89±0.01)％,与对照组(0μmo/L)比较差异具有统计学意义(P＜0.05).Transwell侵袭实验显示,低剂量藤黄酸可抑制LoVo细胞侵袭,其中对照组(0 μmo/L)穿膜细胞数为(120.50±8.69)个,实验组的(1μmol/L)穿膜细胞数为(69.83±4.75)个,两组比较差异有条件下意义(P＜0.05).Western Blot结果显示,GA剂量1、2、4μmol/L作用LoVo细胞后MMP-2蛋白表达水平分别下降48.67％、74.72％、82.70％,与对照组(0μmo/L)比较差异有统计学意义.结论 藤黄酸可抑制LoVo细胞增殖及其侵袭能力,其作用机制可能与降低LoVo细胞中MMP-2蛋白表达有关.

abstractsObjective To investigate the effects of gambogic acid on proliferation,invasion and protein expression of MMP-2 in LoVo human colon cancer cell line.Methods The CCK-8 assay was used to determine the anti-proliferation ability.Transwell chamber assay was used to study the invasion of LoVo colon cancer cells.Western Blot was applied to examine the protein expression of MMP-2.Results CCK-8 assay showed that after cells treated with 1,2,4 μmol/L GA for 24 h,48 h the inhibition rates were(0.16±0.11)％,(0.24±0.08)％,(0.58±0.01)％,(0.67±0.03)％,(0.79±0.01)％ and (0.27±0.05)％,(0.69±0.09)％,(0.85±0.01)％,(0.87±0.01)％,(0.89±0.01)％,and there had statistically significant differences between control group (0 μmo/L) with experimental group (P＜0.05).Gambogic acid can effectively inhibit LoVo cells invasion at a low-dose concentration,which the numbers of cells that digested the matrigel in control group (0 μmo/L)was 120.50± 8.69 and 69.83 ±4.75 in experimental group (1 μnol/L),P＜ 0.05).Western Blot revealed that Gambogic acid can down-regulate the expression of MMP-2,which the levels of down-regulating the expression of MMP-2 after treated with 1,2,4 umol/L GA were 48.67％,74.72％and 82.70％ (P＜0.05) Conclusion Gambogic acid can effectively inhibit the growth and invasion of the LoVo cells,which may be related with down-regulating the expression of MMP-2.