摘要目的：探讨生姜醇提取物对预处理肝脏局部缺血再灌注损伤大鼠的肝保护作用。方法将大鼠按随机数字表法分为假手术组，模型组，生姜醇提取物100、200、400、600 mg/kg预处理组。生姜醇提取物预处理组分别灌胃100、200、400、600 mg/kg生姜醇提取物溶液，假手术组、模型组灌胃等体积生理盐水，1次/d，连续给药2周后造模。除假手术组外，其余各组大鼠制备肝脏缺血再灌注模型，再灌注2 h后，检测各组大鼠肝组织超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)水平；检测血清谷氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、乳酸脱氢酶(LDH)浓度；HE染色观察肝组织病理学改变，TUNEL染色观察肝组织细胞凋亡情况。结果生姜醇提取物100、200、400、600 mg/kg预处理组大鼠肝组织SOD[(7.37±1.89)U/mg、(10.18±2.42)U/mg、(10.50±2.31)U/mg、(11.17±2.83)U/mg比(5.61±1.72)U/mg]、 GSH-Px[(11.31±2.64)U/mg 、(13.07±3.72)U/mg 、(14.63±3.40)U/mg 、(15.14±3.81)U/mg 比(10.92±2.25)U/mg]水平较模型组升高(P＜0.05或 P＜0.01)，MDA 水平[(8.53±2.05)nmol/mg、(5.44±1.37)nmol/mg、(5.20±1.18)nmol/mg、(4.96±1.42)nmol/mg比(10.26±2.27)nmol/mg]较模型组降低(P＜0.05或 P＜0.01)；血清 ALT[(974.62±341.53)U/L、(621.83±214.37)U/L、(553.75±173.48)U/L、(517.92±204.26)U/L比(1429.27±618.33)U/L]、AST[(624.17±148.62)U/L、(456.83±127.35)U/L、(407.54±141.49)U/L、(361.90±104.73)U/L 比(957.61±346.82)U/L]、LDH[(1082.49±312.35)U/L、(897.33±242.76)U/L、(828.42±265.84)U/L、(776.58±182.47)U/L比(1385.61±543.74)U/L]水平较模型组降低(P＜0.05或P＜0.01)；生姜醇提取物各剂量预处理组大鼠肝组织病理损伤及细胞凋亡情况均较模型组有一定的改善。结论生姜醇提取物可有效减轻自由基损伤、改善肝功能、减轻肝细胞损伤和细胞凋亡，对肝脏局部缺血再灌注损伤具有保护作用。

abstractsObjective To investigate the protective effects of the ethanol extract of Zingiber officinale against focal hepatic ischemia-reperfusion injury in rats and itspossible mechanism.Methods The rats were randomly divided into six groups: sham operation group, model control group, ethanol extract of Zingiber officinale 100, 200, 400 and 600 mg/kg per-treated groups. The rats in the sham operation group and the model control group were given equal volume of saline by intragastric administration, once a day; two weeks later, the model were made. Except the sham operation group, the focal hepatic ischemia-reperfusion rats models were established in other groups. Two hours after ischemic-reperfusion, the activity of SOD/GSH-Px and the content of MDA in hepatic tissue were determined; the contents of ALT, AST and LDH in serum were determined; the histopathological changes and hepatocyte apoptosis were observed.Results Compared with the model control group, the activity of SOD in hepar tissue of Ethanol extract of Zingiber officinale 100, 200, 400 and 600 mg/kg per-treated groups (7.37 ± 1.89 U/mg, 10.18 ± 2.42 U/mg, 10.50 ± 2.31 U/mg, 11.17 ± 2.83 U/mgvs. 5.61 ± 1.72 U/mg) were significantly increased (P<0.05,P<0.01), the activity of GSH-Px (11.31 ± 2.64 U/mg, 13.07 ± 3.72 U/mg, 14.63 ± 3.40 U/mg, 15.14 ± 3.81 U/mgvs. 10.92 ± 2.25 U/mg) were significantly increased (P<0.05,P<0.01), and the content of MDA (8.53 ± 2.05 nmol/mg, 5.44 ± 1.37 nmol/mg, 5.20 ± 1.18 nmol/mg, 4.96 ± 1.42 nmol/mgvs. 10.26 ± 2.27 nmol/mg) were significantly decreased (P<0.05,P<0.01); the content of ALT in serum of the ethanol extract of Zingiber officinale 100, 200, 400 and 600 mg/kg per-treated groups (974.62±341.53 U/L, 621.83 ± 214.37 U/L, 553.75 ± 173.48 U/L, 517.92 ± 204.26 U/Lvs. 1 429.27 ± 618.33 U/L), AST (624.17 ± 148.62 U/L, 456.83 ± 127.35 U/L, 407.54 ± 141.49 U/L, 361.90 ± 104.73 U/Lvs. 957.61 ± 346.82 U/L), LDH (1 082.49 ± 312.35 U/L, 897.33 ± 242.76 U/L, 828.42 ± 265.84 U/L, 776.58 ± 182.47 U/Lvs. 1 385.61 ± 543.74 U/L) were all significantly decreased(P<0.05,P<0.01); and the histopathological changes and the hepatocyte apoptosis were significantly improved compared with the model control group.Conclusions The ethanol extract of Zingiber officinale could effectively reduce the damage of free radical, improve hepatic function, inhibit the histopathological changes and hepatocyte apoptosis, suggesting that the ethanol extract of Zingiber officinale had protective effects against focal hepatic ischemia-reperfusion injury in rats.