摘要目的 建立同时测定大血藤中原儿茶酸、红景天苷和绿原酸含量的HPLC法.方法 色谱柱为Waters XSELECT CSH C18(150 mm×4.6 mm,5μm),以乙腈-甲醇-0.2%磷酸水为流动相进行梯度洗脱,体积流量0.8 ml/min,检测波长260 nm,柱温35℃.结果 原儿茶酸、红景天苷和绿原酸分别在0.0020~0.0120、0.0600~0.3602和0.0750~4.5006 mg/ml内呈良好线性关系,平均加样回收率分别为98.01%、98.53%、101.10%,RSD分别为0.07%、0.12%、0.92%.结论 该方法操作简便、准确、重复性好,可为大血藤质量控制提供依据.

abstractsObjective To establish a method for the determination of protocatechuic acid, salidroside, and chlorogenic acid in Sargentodoxa cuneata. Methods The separation was performed on a Waters XSELECT CSH C18 (150 mm × 4.6 mm, 5 μm) with methanol-acetonitrile-0.2 % phosphoric acid as the mobile phase in a gradient elution at a flow rate of 0.8 ml/min. The detection wavelength was 260 nm and the column temperature was 35 ℃. Results The linear ranges of protocatechuic acid, salidroside, and chlorogenic acid were 0.0020-0.0120, 0.0600-0.3602, 0.0750-4.5006 mg/ml, respectively. The average recoveries were 98.01% (RSD=0.07%), 98.53 % (RSD=0.12%), and 101.10 % (RSD=1.92%), respectively. Conclusions The method is simple, accurate, and highly reproducible, which could provide the scientific evidence for the quality control of Sargentodoxa cuneata.