摘要目的:采用超高效液相色谱-线性离子阱/静电场轨道阱高分辨组合质谱技术(ultra-performance liquid chromatography-linear ion trap/orbitraphigh resolution mass spectrometry, UHPLC-LTQ-Orbitrap)分析异类叶升麻苷在大鼠尿液中的代谢产物，并归纳其代谢途径。方法:将大鼠按随机数字表法分为给药组、空白组。给药组大鼠灌胃异类叶升麻苷生理盐水溶液100 mg/kg，空白组灌胃等体积生理盐水。连续收集大鼠尿液12 h，经纯化处理后，采用Thermo Scientific BDS Hypersil C18色谱柱(2.1 mm×150 mm，2.4 μm)，以0.1%甲酸水-乙腈为流动相，梯度洗脱，流速0.3 ml/min，柱温30 ℃。在负离子检测模式下对给药组及空白组尿液进行分析。结果:通过分析高分辨质谱提供的准分子离子峰和碎片离子信息，结合对照品与文献报道结果，从给药组大鼠尿液样品中共鉴定得到8个代谢产物，主要代谢途径为葡萄糖醛酸化、水解、甲基化、磺酸化。结论:应用UHPLC-LTQ-Orbitrap高分辨液质联用技术可分析异类叶升麻苷在大鼠体内主要的代谢产物和代谢途径，为其进一步的药效及药理机制研究提供参考。

abstractsObjective:To analyze the metabolites of isoacteoside in rat's urine after oral administration by UHPLC-LTQ-Orbitrap and then summarize its metabolic pathways.Methods:The rats were randomly divided into treatment and control groups. Isoacteoside dissolved in saline was orally administered to the rats in the treatment group witht a single dose of 100 mg/kg. At the same time, saline was orally administered to the control group with the same volume. The urine samples were collected for 12 h and then purified. Sample analyses were performed on a Thermo Scientific BOS Hypersil C18 column (2.1 mm × 150 mm, 2.4 μm), the mobile phase consisted of water containing 0.1% formic acid-acetonitrile in a gradient program, the flow rate was set at 0.3 ml/min and the column was maintained at 30 ℃. The urine samples of the treatment group and control groups were detected with negative ion mode.Results:The metabolites were identified according to their protonated molecular ions and fragment ions and by comparing the mass data with that of reference standards and the published data. In total, 8 metabolites of isoacteoside were detected and identified in the urine samples of treatment group and the major metabolic pathway of isoacteoside included glucuronide conjugation, dehydroxylation, hydrolyzation, methyl conjugation and sulphate conjugation.Conclusions:UHPLC-LTQ-Orbitrap could be used to analyze the main metabolites and metabolic pathways of isophylloside in rats, which can provide references for further studies on pharmacodynamics and pharmacological mechanisms.