摘要目的:研究淫羊藿不同炮制品的细胞毒性，以及其毒性的物质基础。方法:采用磺酰罗丹明B（SRB）比色法考察淫羊藿不同炮制品对HaCaT细胞增殖的影响，并基于灰色关联度分析法建立HPLC指纹图谱-毒效数据谱效关系，确定其毒性成分及炮制方法。结果:淫羊藿不同炮制品的半抑制浓度（IC 50）值依次为醋炙>羊脂油炙>生品>单炒>盐炙>酒炙，12个特征色谱峰中3号峰（木兰花碱，关联度为0.870）和6号峰（朝藿定C，关联度为0.851）与毒性参考值关联度较高。 结论:醋炙、羊脂油炙均具有减毒作用，木兰花碱、朝藿定C可能是淫羊藿的主要毒性成分，本研究可为淫羊藿炮制减毒的工艺优化提供依据。

abstractsObjective:To explore the effects of different processed products of Epimedii Folium on cytotoxicity and the material basis of toxicity. Methods:By using the SRB method to investigate the effects of different processed products of Epimedii Folium on the proliferation of HaCaT cells; and based on the grey correlation analysis method to establish the data spectrum effect relationship of HPLC fingerprint spectrum-toxicity so as to determine the toxic components and processing methods. Results:The value of cytotoxicity IC 50 of different processed products of Epimedii Folium is as follow: vinegar fried> oil fried > original > single fried > salt fried > wine fried. Among the 12 characteristic chromatographic peaks, Peak No.3 (magnoflorine, correlation value: 0.870) and Peak No.6 (epimedin C, correlation value: 0.851) are highly correlated with the toxicity value. Conclusions:Both vinegar fried and oil fried Epimedii Folium have the effect of reducing toxicity. Magnoflorine and epimedin C may be the main toxic components in Epimedii Folium. The study provides scientific basis for the research on the process optimization of Epimedii Folium concocting to reduce toxicity.