摘要目的:建立胡椒标准汤剂超高效液相图谱（UPLC）特征图谱及胡椒碱含量测定方法。方法:按传统煎煮方法制备15批胡椒标准汤剂冻干粉，测定其出膏率范围，建立标准汤剂UPLC特征图谱，采用高分辨质谱及对照品对共有峰进行指认，以共有峰面积为依据，采用熵权法对各峰权重进行比较，采用聚类分析和灰色关联度法进行关联性分析和相似度评价；同时建立胡椒饮片和标准汤剂冻干粉中胡椒碱含量测定方法，并计算转移率。结果:15批胡椒标准汤剂冻干粉出膏率范围为10.4%～16.8%，均值为14.0%；胡椒碱含量范围为12.2~30.0 mg/g，均值为18.5 mg/g，转移率范围为4.0%~7.8%，均值为5.8%，所建立的特征图谱标定了6个共有峰，通过高分辨质谱分析及对照品定位，确认峰1为N-反式阿魏酰酪胺，峰3为胡椒碱，相似度为0.959~1.000，通过聚类分析与灰色关联度分析发现，胡椒质量与产地之间差异较小。结论:本研究建立了胡椒标准汤剂冻干粉的特征图谱和含量测定方法，可为胡椒标准汤剂或其衍生产品的质量检测和控制提供依据。

abstractsObjective:To establish UPLC characteristic spectrum of Hujiao standard decoction and the determination method for the content of piperine.Methods:15 batches of freeze-dried powder of Hujiao standard decoction were prepared according to the traditional decocting method. The range of paste yield was determined, and the UPLC characteristic spectrum of the standard decoction was established. High-resolution mass spectrometry and control products were used to identify common peaks. Based on the common peak area, the weights of each peak were compared using entropy weight method, and correlation analysis and similarity evaluation were conducted using clustering analysis and grey correlation method; a method for determining the content of piperine in Hujiao decoction pieces and freeze-dried powder of standard decoction was simultaneously established, and the transfer rate was calculated.Results:The extraction rate of 15 batches of freeze-dried powder of Hujiao standard decoction ranged from 10.4% to 16.8%, with an average of 14.0%. The content of piperine ranged from 12.2 to 30.0 mg/g, with an average of 18.5 mg/g, and the transfer rate ranged from 4.0% to 7.8%, with an average of 5.8%. Six common peaks were identified in the established characteristic spectrum and identified by high-resolution mass spectrometry and control products respectively. Peak 1 was N-trans-feruloyltyramine, peak 3 was piperine and the similarity was 0.959-1.000. Clustering analysis and grey correlation analysis showed that there was little difference between quality of Piperis Fructus and origins.Conclusion:In this study, the characteristic spectrum and content determination method of freeze-dried powder of Hujiao standard decoction are established, which can provide references for quality detection and control of Hujiao standard decoction or its derivative products.