摘要Stem/progenitor cells differentiate into different cell lineages during organ development and morphogenesis.Signaling pathway networks and mechanotransduction are important factors to guide the lineage commitment of stem/progenitor cells during craniofacial tissue morphogenesis.Here,we used tooth root development as a model to explore the roles of FGF signaling and mechanotransduction as well as their interaction in regulating the progenitor cell fate decision.We show that Fgfr1 is expressed in the mesenchymal progenitor cells and their progeny during tooth root development.Loss of Fgfr1 in Gli1+progenitors leads to hyperproliferation and differentiation,which causes narrowed periodontal ligament(PDL)space with abnormal cementum/bone formation leading to ankylosis.We further show that aberrant activation of WNT signaling and mechanosensitive channel Piezo2 occurs after loss of FGF signaling in Gli1-CreER;Fgfr1fl/flmice.Overexpression of Piezo2 leads to increased osteoblastic differentiation and decreased Piezo2 leads to downregulation of WNT signaling.Mechanistically,an FGF/PIEZO2/WNT signaling cascade plays a crucial role in modulating the fate of progenitors during root morphogenesis.Downregulation of WNT signaling rescues tooth ankylosis in Fgfr1 mutant mice.Collectively,our findings uncover the mechanism by which FGF signaling regulates the fate decisions of stem/progenitor cells,and the interactions among signaling pathways and mechanotransduction during tooth root development,providing insights for future tooth root regeneration.