摘要Natural products are the prominent sources of drugs to combat various infectious diseases. The rapid progress of multi-resistance and pan-resistant pathogens to various medicines represents today a major challenge. The search for novel natural products requires a quick and efficient approach to distinguish novel compounds from the known ones, a process called dereplication. Dereplication strategy is generic and time-saving, avoids isolation/purification of known compounds, enables an efficient liquid chromatography peak annotation of most of the studied compounds and can be well adapted for plant chemotaxonomy, phytochemical screening and metabolite profiling. In this review, recent developments of hyphenated techniques towards dereplication of active compounds, chemotaxonomy, metabolite profiling and rapid detection of novel compounds in medicinal plant extracts are presented.