摘要Objective:To evaluate the effects of four different concentrations ofBacillus subtilis (B. subtilis) andBacillus circulance (B. circulance) (1×104, 2×104, 3×104 and 4×104CFU/g) on growth and resistance of three spot gourami (Trichopodus trichopterus) for a period of 30 days. <br> Methods: During this study, experimental fish were fed with supplemented diets.Bacillus probiotics with concentrations of 1×104 to 4×104CFU/g were administered to improve the growth performance and larval resistance to challenge tests containing acidic pH and basic pH, ammonia and salinity tests. <br> Results: The addition ofB. subtilis andB. circulance to the diet did not increase larval growth rate, but increased larval resistance against the challenge tests. <br> Conclusions: The results of this study investigated the positive effects ofB. subtilis andB. circulance onTrichopodus trichopterus resistance time to the challenge test.