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Parasitic nematodes of amphibians from Lombok Island, Indonesia with description ofCamallanus senaruensis sp. nov. andMeteterakis lombokensis sp. nov.

摘要Objective:To determine and describe the nematode species of amphibians collected from Lombok Island, West Nusa Tenggara, Indonesia. Methods: The materials examined were found in the intestines of twenty-fourFejervarya cancrivora (F. cancrivora), sixteenFejervarya verruculosa, sixDuttaphyrnus melanostictus (D. melanostictus) from Senaru and Gangga District, Lombok Island on April 2015. The amphibian hosts were collected by hand. Before observing the nematodes, the hosts were anesthesized to death with chloroform. The ventral of the host was opened by longitudinal incision and the internal organs were removed, placed separately in the Petri-dish and then examined under a dissecting microscope. The nematodes found were fixed with warm 70% alcohol. The specimens for light microscope observation were cleared in glycerol and mounted in the same solution, and for theSEM Jeol scanning electron microscope 5310 low vacuumwere re-fixed in caccodylate buffer and glutaraldehyde, dehydrated through a graded series of alcohol and vacuum-dried usingTAITEC Vacuum-96Nitrogen, prior to attaching to stubs with double sided cello-tape, coated with gold 400 ? thickness in an Eico I-B2 ion coater. Drawings were made with the aid of a drawing tube attached to a Nikon compound microscope. Measurements were given in micrometers (μm) as the average, followed by the range in parentheses, unless otherwise stated. Results:Two new species of nematodes were found and described herein:Camallanus senaruensis sp. nov., andMeteterakis lombokensis sp. nov., parasitic in the intestine ofF. cancrivoraandD. melanostictus, respectively.C. senaruensis differs from other congeners in having a bluntly rounded tip of tail in the male and female, the structure of trident and having teeth in the buccal capsule.Meteterakis lombokensis differs from other previously described species in having no vulval flap, has a strongly widened proximal end of spicules, forming a cup shaped, and the number of caudal papillae. Others species found areMeteterakis singaporensis fromD. melanostictus, andChabaudus rauschi (Ch. rauschi) from sixteenF. cancrivoraand eightFejervarya verruculosa. Conclusions: The species of parasitic nematodes from Lombok Island are common in amphibians, butCamallanus,Meteterakis singaporensis,Ch. rauschi are the new record in Indonesia. The dominant species found in Lombok Island isCh. rauschi.

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作者单位 Zoological Division of Research Center for Biology-Indonesian Institute of Sciences, Widyasatwaloka Building, Jl. Raya Jakarta Bogor Km 46, Cibinong 16911, Indonesia [1]
DOI 10.12980/jclm.4.2016J6-181
发布时间 2016-09-29(万方平台首次上网日期,不代表论文的发表时间)
基金项目
Keanekaragaman dan Potensi Biota Nusa Tenggara”, sponsored by Indonesian Institute of Sciences(the project “DIPA of Research Centre for Biology 011/2015)
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