• 医学文献
  • 知识库
  • 评价分析
  • 全部
  • 中外期刊
  • 学位
  • 会议
  • 专利
  • 成果
  • 标准
  • 法规
  • 临床诊疗知识库
  • 中医药知识库
  • 机构
  • 作者
热搜词:
换一批
论文 期刊
取消
高级检索

检索历史 清除

医学文献>>
  • 全部
  • 中外期刊
  • 学位
  • 会议
  • 专利
  • 成果
  • 标准
  • 法规
知识库 >>
  • 临床诊疗知识库
  • 中医药知识库
评价分析 >>
  • 机构
  • 作者
热搜词:
换一批

Effect of macrophage polarization regulated by miR-29b, B7H3 on CD4+T cell differentiation in asthma

摘要Objective: To explore the mechanism that miR-29b and B7H3 regulate the polarization of macrophages and thus affect the differentiation of CD4+T. Methods: 1. PBMC was extracted from peripheral blood mononuclear cells of children with asthma and normal children in the affiliated Children's Hospital of Soochow University, and RNA was extracted and reverse transcribed. The expression of miR-29b and B7H3mRNA was determined by real-time quantitative polymerase chain reaction (Q-PCR). The family history of asthma and history of allergic diseases were collected. 2.THP-1 cells were induced into macrophages, miR-29b interference, miR-29b overexpression and normal control were induced by LV526, LV527 and NC virus infection. After 24 hours of culture, the cells were collected to detect the expression of STAT3 and B7H3 genes and proteins. 3. It was verified that STAT3 was the target gene of miR-29b: after inoculating THP-1 cells and culturing with PMA with final concentration of 50ng/ml for 6 hours, the macrophages without PMA were cultured for 24 hours, then the macrophages infected by LV528, LV529 and NC virus were induced to form miR-29b interference, miR29b overexpression and normal control group. Luciferase analysis was performed at 48 hours to verify that STAT3 was the target gene of miR-29b. STAT3- 3'UTR luciferase reporter gene plasmids were constructed and divided into three groups: "miR-29b+ STAT3- 3'UTR", "miR-29b+STAT3-mut-3'UTR" and "miR-29b+luciferase empty load". 4. Macrophages with different treatments were co-cultured with initial T cells for 3 days. The relative expressions of T-bet, GATA3 and ROR-γt were detected by Q-PCR. Result: 1. The incidence of allergic disease in the acute attack group (68%) was higher than that in the other two groups (34.8%, 33.3%), and the family history of asthma in the normal group (0%) was much lower than that in the other two groups (52%, 60.9%). The difference was statistically significant (P<0.05). 2.The expression of B7H3 in PBMC in acute attack group was higher than that in non-acute attack group and normal group. The expression of miR-29b in PBMC in normal group was significantly higher than that in non-acute attack group and acute attack group (P< 0.0001). The expression of miR-29b in non-acute attack group was significantly higher than that in acute attack group (P= 0.007). 3. After silencing the expression of miR-29b, IL-4Rα, IL-4, IL-5, IL-13 and CD206 of macrophages increased significantly, while IFN- γ decreased, suggesting that miR-29b can promote the polarization of macrophages to M2. 4. The overexpression of miR-29b, STAT3 and B7H3 gene and protein level in macrophages decreased, while the increase of miR-29b, STAT3 and B7H3 gene and protein expression was inhibited. 5. There was a significant positive correlation between the expression of STAT3 and B7H3mRNA in macrophages (r = 0.9737, P< 0.0001). 6. STAT3 is the target gene of miR-29b. 7. Co-culture of macrophages with CD4+T cells can promote the differentiation of primary T cells, namely Th 0 cells, into Th2, and the promoting effect of macrophages with down-regulation of miR-29b is more obvious. Conclusion: The expression of miR-29b in PBMC of children with asthma is lower than that of normal children, while the expression of B7H3 is higher than that of normal children. It is speculated that miR-29b has a protective effect on children with asthma, while B7H3 aggravates the inflammatory response. Down-regulation of miR-29b, in macrophages can promote macrophages to M2 polarization, increase the expression of B7H3 and STAT3 in macrophages, make Th0 cells differentiate into Th2 cells, and aggravate the inflammatory response in patients with asthma.

更多
广告
作者 Yue-Yue Wang [1] Wei Ji [1] Zheng-Rong Chen [1] Wen-Jing Gu [1] 学术成果认领
作者单位 Children's Hospital affiliated to Soochow University,Suzhou 215003,Jiangsu [1]
栏目名称 Original article
发布时间 2021-05-17
基金项目
National Natural Science Foundation of China
提交
  • 浏览5
  • 下载0
海南医科大学学报(英文版)

加载中!

相似文献

  • 中文期刊
  • 外文期刊
  • 学位论文
  • 会议论文

加载中!

加载中!

加载中!

加载中!

特别提示:本网站仅提供医学学术资源服务,不销售任何药品和器械,有关药品和器械的销售信息,请查阅其他网站。

  • 客服热线:4000-115-888 转3 (周一至周五:8:00至17:00)

  • |
  • 客服邮箱:yiyao@wanfangdata.com.cn

  • 违法和不良信息举报电话:4000-115-888,举报邮箱:problem@wanfangdata.com.cn,举报专区

官方微信
万方医学小程序
new翻译 充值 订阅 收藏 移动端

官方微信

万方医学小程序

使用
帮助
Alternate Text
调查问卷