摘要Objective: To study the antiviral activity of Guanhuang Ganmao Keli on human respiratory syncytial virus (RSV) in vitro. Methods: The Guanhuang Ganmao Keli was dissolved in pure water and filtered by a 0.22 micron filter to get solution. Cyclopiazonic acid (CPA) was used as positive control. The toxicity of Guanhuang Ganmao Keli on Hep-2 cells was tested by cell counting kit 8 (CCK-8). The protective effect of Guanhuang Ganmao Keli on RSV was evaluated under the highest toxic concentration. Results: The TC50 and EC50 of Guanhuang Ganmao Keli is 0.647 mg/ml and 0.014 mg/ml, respectively. Guanhuang Ganmao Keli showed significant antiviral effect when added 0、2、4、6 and 8h post-infection. Conclusions: Guanhuang Ganmao Keli is an effective antiviral agent on RSV in vitro.