摘要The gut microbiome is closely associated with human health and the development of diseases.Isolating,characterizing,and identifying gut microbes are crucial for research on the gut microbiome and essential for advancing our understanding and utilization of it.Although culture-independent approaches have been developed,a pure culture is required for in-depth analysis of disease mechanisms and the development of biotherapy strategies.Currently,microbiome research faces the challenge of expanding the existing database of culturable gut microbiota and rapidly isolating target microorganisms.This review examines the advancements in gut microbe isolation and cultivation techniques,such as culturomics,droplet microfluidics,phenotypic and genomics selection,and membrane diffusion.Furthermore,we evaluate the progress made in technology for identifying gut microbes considering both non-targeted and targeted strategies.The focus of future research in gut microbial culturomics is expected to be on high-throughput,automation,and integration.Advancements in this field may facilitate strain-level investigation into the mechanisms underlying diseases related to gut microbiota.