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CRISPR/Cas9 nickase mediated signal amplification integrating with the trans-cleavage activity of Cas12a for highly selective and sensitive detection of single base mutations

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学术成果认领

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摘要Dear Editor,Mutations in genomic sequences exhibit a strong correlation with various pathological processes of cancers[1].Currently,the next-generation sequencing technique[2]and polymerase chain reaction(PCR)were the established benchmarks for analyzing DNA mutations.However,the two methods necessitate intricate experimental preparation,costly instrumentation,and skilled personnel,making them challenging for rapid mutations analysis.More importantly,these methods lack adequate accuracy for one base mutations analysis[3].Therefore,the development of a reliable and exceptionally sensitive mutation analysis approach holds immense importance in cancer diagnosis and treatment.

作者 Xiao-Wen Fan ^[1] Zi-Fan Gao ^[1] Dong-Dong Ling ^[2] De-Hui Wang ^[2] Ying Cui ^[2] Hui-Qun Du ^[2] Chun-Lin Li ^[3] Xing Zhou ^[4] 学术成果认领

作者单位 Jinling Clinical Medical College,Nanjing University of Chinese Medicine,Nanjing 210002,China ^[1] Department of Orthopedics,Affiliated Hospital of Medical School,Jinling Hospital,Nanjing University,Nanjing 210002,China ^[2] Department of Health Medicine,the Eighth Medical Center of PLA General Hospital,Beijing 100093,China ^[3] Jinling Clinical Medical College,Nanjing University of Chinese Medicine,Nanjing 210002,China;Department of Orthopedics,Affiliated Hospital of Medical School,Jinling Hospital,Nanjing University,Nanjing 210002,China ^[4]

关键词 Cas9 nickase CRISPR-Cas12a Polymerase Single base mutations

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LETTER TO THE EDITOR

DOI 10.1186/s40779-024-00530-x

发布时间 2025-07-04（万方平台首次上网日期，不代表论文的发表时间）

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