摘要More than 100 types of chemical modifications in RNA have been well documented. Recently,several modifications,such as N6-methyladenosine(m6A),have been detected in mRNA, opening the window into the realm of epitranscriptomics.The m6A modification is the most abun-dant modification in mRNA and non-coding RNA(ncRNA).At the molecular level,m6A affects almost all aspects of mRNA metabolism, including splicing, translation, and stability, as well as microRNA (miRNA) maturation, playing essential roles in a range of cellular processes. The m6A modification is regulated by three classes of proteins generally referred to as the""writer"(ade-nosine methyltransferase),""eraser"(m6A demethylating enzyme),and""reader"(m6A-binding pro-tein).The m6A modification is reversibly installed and removed by writers and erasers,respectively. Readers,which are members of the YT521-B homology(YTH)family proteins,selectively bind to RNA and affect its fate in an m6A-dependent manner.In this review,we summarize the structures of the functional proteins that modulate the m6A modification, and provide our insights into the m6A-mediated gene regulation.