摘要目的 探讨骨髓间充质干细胞(bone marrow mesenchynal stem cells,BMSCs)来源的神经干样细胞(neural stem-iike cells derived from bone marrow mesenchymal stem cells,MS-NSCs)对急性重症一氧化碳中毒大鼠脑白质结构的影响.方法 40只200～250 g SD(sprague Dawley)大鼠随机分成正常对照组、中毒对照组、BMSCs治疗组、MS-NSCs治疗组(每组10只).先体外分离大鼠BMSCs进行MS-NSCs定向诱导分化,并作BrdU标记后经左颈内动脉移植入急性重度一氧化碳中毒复苏24 h的大鼠脑内,5周后病理学上评价脑白质结构的重塑.结果 5周后,细胞移植能够改善白质结构的有序性、致密性及脱髓鞘变化,同时在疏松白质内检测到BrdU阳性细胞,且在MS-NSCs移植组中此阳性细胞数更多(P＜0.05).结论 MS-NSCs能够重塑急性重症一氧化碳中毒大鼠的脑白质结构,而且可能是促进脑功能恢复的理想种子细胞.

abstractsObjective To investigate changes in cerebral white matter after transplantation of neural stem like cells (MS-NSCs) derived from the bone marrow mesenchymal stem cells, in rats with severe acute carbon monoxide poisoning. Method Forty Sprague-Dawley rats weighing 200 ～ 250 g were divided randomly into 4 groups: the normal control group, the poisoned control group, the BMSCs transplanted group and the MS-NSCs transplanted group (each group 6 rats). BMSCs were harvested from whole bone marrow in vitro, and then differentiated into MS-NSCs under the cock tail of certain growth factors, followed by BrdU labelling. The seed cells were infused into the brain via the left internal carotid artery 24 hours post poisoning. Remodelling of cerebral white matter was assessed using H & E staining, myelin staining and immunohistochemitry assay after 5 weeks later. Results Cellular transplantation improved the compactness and orderliness of cerebral white matter. BrdU-positive cells were found in the focal insulted areas of sparse white matter; and greater numbers of Brdu-Positive ceus were observed in the MS-NSCs group thar in the BMSCs group (P ＜0.05). Conclusions MS-NSCs participates in the remodeling of cerebral white matter in rats with severe acute carbon monoxide poisoning, and shows promising evidence as seed cells transplanted for brain tissue regeneration.